Fengycin produced by Bacillus subtilis XF-1 plays a major role in the biocontrol of Chinese cabbage clubroot via direct effect and defense stimulation
文献类型： 外文期刊
作者： He, Pengjie 1 ; Cui, Wenyan 1 ; Munir, Shahzad 1 ; He, Pengbo 1 ; Huang, Ruirong 3 ; Li, Xingyu 1 ; Wu, Yixin 1 ; Wang, Yuehu 4 ; Yang, Jing 1 ; Tang, Ping 1 ; He, Yueqiu 1 ; He, Pengfei 1 ;
作者机构： 1.Yunnan Agr Univ, State Key Lab Conservat & Utilizat Bioresources Yu, Kunming, Peoples R China
2.Guizhou Univ Tradit Chinese Med, Guiyang, Peoples R China
3.Jiangxi Acad Agr Sci, Plant Protect Inst, Nanchang, Peoples R China
4.Chinese Acad Sci, Kunming Inst Bot, Yunnan Key Lab Fungal Divers & Green Dev, Kunming, Peoples R China
5.Yunnan Agr Univ, State Key Lab Conservat & Utilizat Bioresources Yu, Kunming, Yunnan, Peoples R China
关键词： Bacillus subtilis; colonization; fengycin; foliar application; Plasmodiophora brassicae Woron; soil-borne disease
期刊名称：JOURNAL OF CELLULAR PHYSIOLOGY （ 影响因子：5.6； 五年影响因子：5.9 ）
ISSN： 0021-9541
年卷期： 2023 年
页码：
收录情况： SCI
摘要： Bacillus subtilis XF-1 is a well-investigated biocontrol agent against the biotrophic Plasmodiophora brassicae Woron., the causal agent of clubroot disease of cruciferous crops. The present study demonstrates that XF-1 could efficiently control clubroot disease via leaf spraying and provides an understanding of the biocontrol mechanisms. High-performance thin-layer chromatography (HTPLC) analysis indicated the presence of fengycin-type cyclopeptides in the supernatant. A ppsB deletion mutant of XF-1 resulted in no fengycin production, significantly reduced the lysis rate of testing spores in vitro and the primary infection rate of root hair in vivo, and decreased the protection value against clubroot disease under the greenhouse conditions. Confocal laser scanning microscopy proved that fengycin was not required for leaf internalization and root colonization. Moreover, the expression level of the ppsB gene in XF-1 was regulated by its cell density in root during interaction with P. brassicae. In addition, the Delta ppsB mutant of XF-1 could not efficiently control disease because it led to a lower activation level of the jasmonic acid and salicylic acid signaling pathways in roots, which are necessary for the plant defense reaction upon pathogen invasion. Altogether, the present study provides a new understanding of specific cues in the interaction between B. subtilis and P. brassicae as well as insights into the application of B. subtilis in agriculture.
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