文献类型： 外文期刊

作者： Sha, Gan ¹ ; Sun, Peng ¹ ; Kong, Xiaojing ¹ ; Han, Xinyu ¹ ; Sun, Qiping ¹ ; Fouillen, Laetitia ⁵ ; Zhao, Juan ¹ ; Li, Yun ¹ ; Yang, Lei ¹ ; Wang, Yin ¹ ; Gong, Qiuwen ¹ ; Zhou, Yaru ¹ ; Zhou, Wenqing ¹ ; Jain, Rashmi ⁶ ; Gao, Jie ⁹ ; Huang, Renliang ¹⁰ ; Chen, Xiaoyang ¹ ; Zheng, Lu ¹ ; Zhang, Wanying ¹ ; Qin, Ziting ¹ ; Zhou, Qi ¹¹ ; Zeng, Qingdong ¹² ; Xie, Kabin ⁹ ; Xu, Jiandi ¹³ ; Chiu, Tsan-Yu ¹¹ ; Guo, Liang ⁹ ; Mortimer, Jenny C. ⁸ ; Boutte, Yohann ⁵ ; Li, Qiang ⁹ ; Kang, Zhensheng ¹² ; Ronald, Pamela C. ⁶ ; Li, Guotian ¹ ;

作者机构： 1.Huazhong Agr Univ, Natl Key Lab Agr Microbiol, Wuhan, Peoples R China

2.Huazhong Agr Univ, Hubei Hongshan Lab, Wuhan, Peoples R China

3.Huazhong Agr Univ, Hubei Key Lab Plant Pathol, Wuhan, Peoples R China

4.Huazhong Agr Univ, Ctr Crop Nanobiotechnol, Wuhan, Peoples R China

5.Univ Bordeaux, Lab Biogenese Membranaire, CNRS, Villenave Dornon, France

6.Univ Calif Davis, Dept Plant Pathol, Davis, CA 95616 USA

7.Univ Calif Davis, Genome Ctr, Davis, CA 95616 USA

8.Joint BioEnergy Inst, Feedstocks Div, Emeryville, CA 94608 USA

9.Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan, Peoples R China

10.Jiangxi Acad Agr Sci, Rice Res Inst, Natl Engn Res Ctr Rice Nanchang, Key Lab Rice Physiol & Genet Jiangxi Prov, Nanchang, Peoples R China

11.BGI Shenzhen, Shenzhen, Peoples R China

12.Northwest A&F Univ, State Key Lab Crop Stress Biol Arid Areas, Yangling, Peoples R China

13.Shandong Acad Agr Sci, Inst Wetland Agr & Ecol, Jinan, Peoples R China

14.Univ Adelaide, Sch Agr Food & Wine, Glen Osmond, SA, Australia

15.Univ Calif Berkeley, Innovat Genom Inst, Berkeley, CA 94720 USA

16.Chengdu Normal Univ, Coll Chem & Life Sci, Sichuan Prov Key Lab Dev & Utilizat Characterist, Chengdu, Peoples R China

17.Anhui Agr Univ, Coll Plant Protect, Hefei, Peoples R China

期刊名称：NATURE （ 影响因子：64.8； 五年影响因子：60.9 ）

ISSN： 0028-0836

年卷期： 2023 年 618 卷 7967 期

页码：

收录情况： SCI

摘要： The discovery and application of genome editing introduced a new era of plant breeding by giving researchers efficient tools for the precise engineering of crop genomes(1). Here we demonstrate the power of genome editing for engineering broad-spectrum disease resistance in rice (Oryza sativa). We first isolated a lesion mimic mutant (LMM) from a mutagenized rice population. We then demonstrated that a 29-base-pair deletion in a gene we named RESISTANCE TO BLAST1 (RBL1) caused broad-spectrum disease resistance and showed that this mutation caused an approximately 20-fold reduction in yield. RBL1 encodes a cytidine diphosphate diacylglycerol synthase that is required for phospholipid biosynthesis(2). Mutation of RBL1 results in reduced levels of phosphatidylinositol and its derivative phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P-2). In rice, PtdIns(4,5)P-2 is enriched in cellular structures that are specifically associated with effector secretion and fungal infection, suggesting that it has a role as a disease-susceptibility factor(3). By using targeted genome editing, we obtained an allele of RBL1, named RBL1(Delta 12), which confers broad-spectrum disease resistance but does not decrease yield in a model rice variety, as assessed in small-scale field trials. Our study has demonstrated the benefits of editing an LMM gene, a strategy relevant to diverse LMM genes and crops.