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Identification and Characterization of Short Crown Root 8, a Temperature-Sensitive Mutant Associated with Crown Root Development in Rice

文献类型： 外文期刊

作者： Hu, Peng 1 ; Wen, Yi 1 ; Wang, Yueying 1 ; Wu, Hao 1 ; Wang, Junge 1 ; Wu, Kaixiong 1 ; Chai, Bingze 1 ; Zhu, Lixin 1 ; Zhan 1 ;

作者机构： 1.China Natl Rice Res Inst, State Key Lab Rice Biol, Hangzhou 310006, Peoples R China

2.Chinese Acad Agr Sci, Agr Genom Inst Shenzhen, Shenzhen 518120, Peoples R China

3.Shenyang Agr Univ, Rice Res Inst, Key Lab Northeast Rice Biol & Breeding, Minist Agr, Shenyang 110866, Peoples R China

4.Jiangxi Acad Agr Sci, Rice Res Inst, Nanchang 330200, Jiangxi, Peoples R China

5.Zhejiang Normal Univ, Coll Chem & Life Sci, Jinhua 321004, Zhejiang, Peoples R China

关键词： crown root; defense response; temperature-sensitive; scr8; rice

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.924； 五年影响因子：6.132 ）

ISSN：

年卷期： 2021 年 22 卷 18 期

页码：

收录情况： SCI

摘要： Crown roots are essential for plants to obtain water and nutrients, perceive environmental changes, and synthesize plant hormones. In this study, we identified and characterized short crown root 8 (scr8), which exhibited a defective phenotype of crown root and vegetative development. Temperature treatment showed that scr8 was sensitive to temperature and that the mutant phenotypes were rescued when grown under low temperature condition (20 degrees C). Histological and EdU staining analysis showed that the crown root formation was hampered and that the root meristem activity was decreased in scr8. With map-based cloning strategy, the SCR8 gene was fine-mapped to an interval of 126.4 kb on chromosome 8. Sequencing analysis revealed that the sequence variations were only found in LOC_Os08g14850, which encodes a CC-NBS-LRR protein. Expression and inoculation test analysis showed that the expression level of LOC_Os08g14850 was significantly decreased under low temperature (20 degrees C) and that the resistance to Xanthomonas oryzae pv. Oryzae (Xoo) was enhanced in scr8. These results indicated that LOC_Os08g14850 may be the candidate of SCR8 and that its mutation activated the plant defense response, resulting in a crown root growth defect.

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