您好，欢迎访问江西省农业科学院 机构知识库！

Combination of Genomics, Transcriptomics Identifies Candidate Loci Related to Cold Tolerance in Dongxiang Wild Rice

文献类型： 外文期刊

作者： Wang, Dianwen 1 ; Xiao, Yulong 1 ; Chen, Hongping 1 ; Huang, Cheng 1 ; Chen, Ping 1 ; Chen, Dazhou 1 ; Deng, Wei 1 ; Wang, Jilin 1 ;

作者机构： 1.Jiangxi Acad Agr Sci, Rice Natl Engn Res Ctr Nanchang, Rice Res Inst, Nanchang 330200, Jiangxi, Peoples R China

关键词： Dongxiang wild rice; seedling cold tolerance; quantitative trait locus; transcriptomics; differentially expressed genes

期刊名称：PLANTS-BASEL （ 影响因子：4.658； 五年影响因子：4.827 ）

ISSN：

年卷期： 2022 年 11 卷 18 期

页码：

收录情况： SCI

摘要： Rice, a cold-sensitive crop, is a staple food for more than 50% of the world's population. Low temperature severely compromises the growth of rice and challenges China's food safety. Dongxiang wild rice (DXWR) is the most northerly common wild rice in China and has strong cold tolerance, but the genetic basis of its cold tolerance is still unclear. Here, we report quantitative trait loci (QTLs) analysis for seedling cold tolerance (SCT) using a high-density single nucleotide polymorphism linkage map in the backcross recombinant inbred lines that were derived from a cross of DXWR, and an indica cultivar, GZX49. A total of 10 putative QTLs were identified for SCT under 4 degrees C cold treatment, each explaining 2.0-6.8% of the phenotypic variation in this population. Furthermore, transcriptome sequencing of DXWR seedlings before and after cold treatment was performed, and 898 and 3413 differentially expressed genes (DEGs) relative to 0 h in cold-tolerant for 4 h and 12 h were identified, respectively. Gene ontology and Kyoto encyclopedia of genes and genomes (KEGG) analysis were performed on these DEGs. Using transcriptome data and genetic linkage analysis, combined with qRT-PCR, sequence comparison, and bioinformatics, LOC_Os08g04840 was putatively identified as a candidate gene for the major effect locus qSCT8. These findings provided insights into the genetic basis of SCT for the improvement of cold stress potential in rice breeding programs.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：江西省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台