Selenium Species Determination in Se-Enriched Grain Crops with Foliar Spray of Sodium Selenite by IP-RP-HPLC-UV-HG-AFS
文献类型： 外文期刊
作者： Wei, Yihua 1 ; Zhang, Jinyan 1 ; Qiu, Shuyan 1 ; Huang, Qingqing 2 ; Yuan, Linfeng 1 ; Chen, Liumeng 3 ; Dai, Tingcan; 1 ;
作者机构： 1.Jiangxi Acad Agr Sci, Inst Qual & Safety & Stand Agr Prod Res, Nanchang 330200, Jiangxi, Peoples R China
2.Minist Agr & Rural Affairs, Agroenvironm Protect Inst, Tianjin 300191, Peoples R China
3.Jiangxi Acad Agr Sci, Inst Appl Agr Microorganism, Nanchang 330200, Jiangxi, Peoples R China
关键词： Se species; Ion-pair HPLC; UV-HG-AFS; Foliar spray; Se-enriched grain crops
期刊名称：FOOD ANALYTICAL METHODS （ 影响因子：3.366； 五年影响因子：3.07 ）
ISSN： 1936-9751
年卷期：
页码：
收录情况： SCI
摘要： A simple, rapid, and accurate method for determination of selenocystine (SeCys(2)), methyl selenocysteine (MeSeCys), selenourea (SeUr), selenite (Se(IV)), selenotmethionine (SeMet), and selenate (Se(VI)) in grain crops was developed, based on the coupling of ion-pair reversed-phase high-performance liquid chromatography (IP-RP-HPLC) and ultraviolet irradiation hydride generation atomic fluorescence spectrometry (UV-HG-AFS). The conditions of IP-RP-HPLC-UV-HG -AFS were studied. The conditions of the pretreatment sample, such as types and dosage of proteinases, were investigated. The detection limits (LODs) for six Se species were between 0.77 and 1.77 mu g center dot L-1. Then, the proposed method has been successfully used to analyze selenium (Se) species in Se-enriched rice paddy (Oryza sativa L.), soybean (Glycine max (Linn.) Merr.), and sweet potato (Ipomoea batatas (L.) Lam.) with foliar application of sodium selenite solution. SeMet was the major Se spices in Se-enriched grain crops, ranged from 77-90% of the total Se contents. The capabilities of Se enrichment were relatively larger differences in three-grain corps as follows: soybean > rice paddy > sweet potato.
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