Knockdown of CYP301B1 and CYP6AX1v2 increases the susceptibility of the brown planthopper to beta-asarone, a potential plant-derived insecticide
文献类型： 外文期刊
作者： Xu, Xueliang 1 ; Li, Xiang 3 ; Liu, Zirong 1 ; Wang, Fenshan 1 ; Fan, Linjuan 1 ; Wu, Caiyun 1 ; Yao, Yingjuan 1 ;
作者机构： 1.Jiangxi Acad Agr Sci, Appl Agr Microorganism Res, Nanchang 330200, Jiangxi, Peoples R China
2.Huazhong Agr Univ, Coll Plant Sci & Technol, Hubei Insect Resources Utilizat & Sustainable Pes, Wuhan 430070, Peoples R China
3.Henan Agr Univ, Coll Plant Protect, Zhengzhou 450000, Peoples R China
关键词： beta-asarone; Brown planthopper; Cytochrome P450 genes; Expression patterns; RNA interference
期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：6.953； 五年影响因子：6.737 ）
ISSN： 0141-8130
年卷期： 2021 年 171 卷
页码：
收录情况： SCI
摘要： The cytochrome P450 monooxygenases of insects play crucial roles in the metabolic detoxification of insecticides. Our previous finding showed that two cytochrome P450 genes, both CYP301B1 and CYP6AX1v2, in the BPH underwent overexpression due to beta-asarone. In this study, we investigated the molecular characteristics, expression patterns and functions of these two cytochrome P450 genes. The results showed that CYP301B1 had the highest expression level in the eggs, while CYP6AX1v2 was expressed in macropterous female adults. Moreover, the expression level of CYP301B1 in the head was higher than that in the integument, fat body and gut. The expression level of CYP6AX1v2 in the fat body and gut was higher than that in head and integument. Importantly, silencing CYP301B1 and CYP6AX1v2 separately could increase the sensitivity, resulting in significant higher mortality of BPH following treatment with beta-asarone. Our findings indicated that CYP301B1 and CYP6AX1v2 could contribute to the resistance of BPH to beta-asarone, and these two genesmay be involved in the detoxification metabolism of beta-asarone in BPH. (C) 2021 Published by Elsevier B.V.
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