文献类型： 外文期刊

作者： Cui, Junjie ² ; Luo, Shaobo ³ ; Niu, Yu ⁴ ; Huang, Rukui ⁵ ; Wen, Qingfang ⁶ ; Su, Jianwen ⁷ ; Miao, Nansheng ⁸ ; He, Wei ¹ ;

作者机构： 1.South China Agr Univ, Coll Hort, Guangzhou, Guangdong, Peoples R China

2.South China Agr Univ, Coll Hort, Minist Agr, Key Lab Biol & Germplasm Enhancement Hort Crops S, Guangzhou, Guangdong, Peoples R China

3.Guangdong Acad Agr Sci, Vegetable Res Inst, Guangzhou, Guangdong, Peoples R China

4.Chinese Acad Trop Agr Sci, Trop Crops Genet Resources Inst, Danzhou, Peoples R China

5.Guangxi Acad Agr Sci, Vegetable Res Inst, Nanning, Peoples R China

6.Fujian Acad Agr Sci, Crops Res Inst, Fuzhou, Fujian, Peoples R China

7.Hunan Vegetable Res Inst, Changsha, Hunan, Peoples R China

8.Jiangxi Acad Agr Sci, Inst Vegetables & Flowers, Nanchang, Jiangxi, Peoples R China

9.Beijing Genom Inst, Shenzhen, Peoples R China

关键词： bitter gourd; restriction site associated DNA (RAD); genetic map; genome assembly; genetic location

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：5.753； 五年影响因子：6.612 ）

ISSN： 1664-462X

年卷期： 2018 年 9 卷

页码：

收录情况： SCI

摘要： Genetic mapping is a basic tool necessary for anchoring assembled scaffold sequences and for identifying QTLs controlling important traits. Though bitter gourd (Momordica charantia) is both consumed and used as a medicinal, research on its genomics and genetic mapping is severely limited. Here, we report the construction of a restriction site associated DNA (RAD)-based genetic map for bitter gourd using an F-2 mapping population comprising 423 individuals derived from two cultivated inbred lines, the gynoecious line 'K44' and the monoecious line 'Dali-11.' This map comprised 1,009 SNP markers and spanned a total genetic distance of 2,203.95 cM across the 11 linkage groups. It anchored a total of 113 assembled scaffolds that covered about 251.32 Mb (85.48%) of the 294.01 Mb assembled genome. In addition, three horticulturally important traits including sex expression, fruit epidermal structure, and immature fruit color were evaluated using a combination of qualitative and quantitative data. As a result, we identified three QTL/gene loci responsible for these traits in three environments. The QTL/gene gy/fffn/ffn, controlling sex expression involved in gynoecy, first female flower node, and female flower number was detected in the reported region. Particularly, two QTLs/genes, Fwa/Wr and w, were found to be responsible for fruit epidermal structure and white immature fruit color, respectively. This RAD-based genetic map promotes the assembly of the bitter gourd genome and the identified genetic loci will accelerate the cloning of relevant genes in the future.