Dietary conjugated linoleic acid modulates morphology, selective immune parameters, and gene expressions in the intestine of grass carp
文献类型： 外文期刊
作者： Kong, Long 1 ; Cheng, Shi-yan 1 ; Xiang, Xiao-jun 1 ; Liu, Wen-shu 2 ; Yu, Deng-hang 1 ; Yang, Yan-ou 3 ; Zhou, Jianch 1 ;
作者机构： 1.Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China
2.Jiangxi Acad Agr Sci, Inst Anim Husb & Vet, Nanchang 330200, Jiangxi, Peoples R China
3.Anhui Agr Univ, Coll Anim Sci & Technol, Hefei 230000, Anhui, Peoples R China
4.Wuhan DBN Aquaculture Technol Co Ltd, Wuhan 430090, Hubei, Peoples R China
关键词： Conjugated linoleic acid; Intestinal morphology; Immune responses; TLR4/NF-kappa B signaling pathway; Ctenopharyngodon idella
期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.581； 五年影响因子：4.851 ）
ISSN： 1050-4648
年卷期： 2019 年 86 卷
页码：
收录情况： SCI
摘要： Conjugated linoleic acid (CLA) has been shown to exhibit anti-inflammatory properties in the intestine in mammals. However, the effect of CLA on intestinal immune response in fish is still unknown. Therefore, a 65-day growth trial was conducted to investigate the effects of dietary conjugated linoleic acid (CLA) on morphology, selective immune parameters, and gene expressions in the intestine of grass carp. Seven isonitrogenous and isolipidic diets were formulated as follows: 0 (control), 0.5 (CLA0.5), 1 (CLA1), 1.5 (CLA1.5), 2 (CLA2), 2.5 (CLA2.5), and 3 (CLA3) g CLA per 100g of feed. Results: showed that dietary supplementation of 1.5-3% CLA significantly (P < 0.05) increased the fold and enterocyte heights in the PI and MI of grass carp. Complement 3 (C3) and immunoglobulin M (IgM) contents in three intestinal segments were significantly (P < 0.05) higher in fish fed with CLA1.5 to CLA2.5 diets compared to fish fed the control diet. CLA1.5 to CLA2.5 diets significantly (P < 0.05) increased the mRNA expression levels of anti-inflammatory cytokines (IL-10 and TGF beta 1) and significantly (P < 0.05) reduced the mRNA expression levels of pro-inflammatory cytokines (IL-l beta, IL-8, and TNF-alpha) in the PI, MI, and DI. This improved expression of anti-inflammatory cytokines and the inhibited expression of pro-inflammatory cytokines in the intestine of grass carp, might be mediated via TLR4/NF-kappa B-signaling pathway. Our results suggested that CLA1.5 to CLA2 diets improved intestinal morphology, increased the expression of anti-inflammatory cytokines, and inhibited the expression of pro-inflammatory cytokines in the intestine of grass carp. In conclusion, dietary supplementation of 1.5%-2% CIA show the anti-inflammatory therapeutic potential in the intestine of grass carp. The anti-inflammatory therapeutic potential of CIA might be mediated via TLR4/NF-kappa B-signaling pathway.
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