文献类型： 外文期刊

作者： Chen, Lunlin ¹ ; Wan, Heping ¹ ; Qian, Jiali ¹ ; Guo, Jianbin ¹ ; Sun, Chengming ¹ ; Wen, Jing ¹ ; Yi, Bin ¹ ; Ma, Chaozh ¹ ;

作者机构： 1.Huazhong Agr Univ, Coll Plant Sci & Technol, Natl Engn Res Ctr Rapeseed, Natl Key Lab Crop Genet Improvement, Wuhan, Hubei, Peoples R China

2.Jiangxi Acad Agr Sci, Crops Res Inst, Jiangxi Prov Key Lab Oil Crops Biol, Natl Ctr Oilcrops Improvement,Nanchang Branch, Nanchang, Jiangxi, Peoples R China

关键词： Brassica napus; cadmium; genome-wide association study; genotypic variations; hyperaccumulator; quantitative trait loci

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：5.753； 五年影响因子：6.612 ）

ISSN： 1664-462X

年卷期： 2018 年 9 卷

页码：

收录情况： SCI

摘要： Cadmium is a potentially toxic heavy metal to human health. Rapeseed (Brassica napus L.), a vegetable and oilseed crop, might also be a Cd hyperaccumulator, but there is little information on this trait in rapeseed. We evaluated Cd accumulation in different oilseed accessions and employed a genome-wide association study to identify quantitative trait loci (QTLs) related to Cd accumulation. A total of 419 B. napus accessions and inbred lines were genotyped with a 60K Illumina Infinium SNP array of Brassica. Wide genotypic variations in Cd concentration and translocation were found. Twenty-five QTLs integrated with 98 single-nucleotide polymorphisms (SNPs) located at 15 chromosomes were associated with Cd accumulation traits. These QTLs explained 3.49-7.57% of the phenotypic variation observed. Thirty-two candidate genes were identified in these genomic regions, and they were 0.33-497.97 kb away from the SNPs. We found orthologs of Arabidopsis thaliana located near the significant SNPs on the B. napus genome, including NRAMP6 (natural resistance-associated macrophage protein 6), IRT1 (iron-regulated transporter 1), CAD1 (cadmium-sensitive 1), and PCS2 (phytochelatin synthase 2). Of them, four candidate genes were verified by qRT-PCR, the expression levels of which were significantly higher after exposure to Cd than in the controls. Our results might facilitate the study of the genetic basis of Cd accumulation and the cloning of candidate Cd accumulation genes, which could be used to help reduce Cd levels in edible plant parts and/or create more efficient hyperaccumulators.