microRNA-14 as an efficient suppressor to switch off ecdysone production after ecdysis in insects
文献类型： 外文期刊
作者： He, Kang 1 ; Xiao, Huamei 2 ; Sun, Yang 3 ; Situ, Gongming 3 ; Xi, Yu 5 ; Li, Fei 1 ;
作者机构： 1.Zhejiang Univ, Minist Agr & Rural Affairs, Key Lab Mol Biol Crop Pathogens & Insects, 866 Yuhangtang Rd, Hangzhou 310058, Zhejiang, Peoples R China
2.Yichun Univ, Coll Life Sci & Resource Environm, Yichun, Peoples R China
3.Nanjing Agr Univ, Dept Entomol, Nanjing, Jiangsu, Peoples R China
4.Jiangxi Acad Agr Sci, Inst Plant Protect, Nanchang, Jiangxi, Peoples R China
5.Chinese Acad Agr Sci, Agr Genomes Inst Shenzhen, Shenzhen, Peoples R China
关键词： Microrna; molting timing; developmental synchrony; ecdysone
期刊名称：RNA BIOLOGY （ 影响因子：4.652； 五年影响因子：6.774 ）
ISSN： 1547-6286
年卷期： 2019 年 16 卷 9 期
页码：
收录情况： SCI
摘要： The precise increase and decrease of hormone ecdysone are critical for accurate development in insects. Most previous works focus on transcriptional activation of ecdysone production; however, little is known about the mechanism of switching off ecdysone biosynthesis after ecdysis. Here, we showed that the precursor microRNA-14 (pre-miR-14) encodes two mature miRNAs in silkworm; both of these two mature miRNAs regulate various genes in the ecdysone-signalling pathway. Bmo-miR-14-5p targets on nine genes whereas Bmo-miR-14-3p targets on two genes in the same pathway. These two mature miRNAs increased immediately after the ecdysis, efficiently suppressing the 20-hydroxyecdysone (20E) biosynthesis, the upstream regulation, and the downstream response genes. Knocking down either of two mature miRNAs or both of them delays moult development, impairing development synchrony in antagomir-treated groups. In addition, overexpressing Bmo-miR-14-5p but not Bmo-miR-14-3p significantly affected the 20E titer and increased the moulting time variation, suggesting that Bmo-miR-14-5p, though it is less abundant, has more potent effects in development regulation than Bmo-miR-14-3p. In summary, we present evidence that a pre-miRNA encodes two mature miRNAs targeting on the same pathway, which significantly improves miRNA regulation efficiencies to programmatically switch off ecdysone biosynthesis.
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