文献类型： 外文期刊
作者： Yao, Jian 1 ; Chen, Qing Long 1 ; Shen, Ai Xi 1 ; Cao, Wen 1 ; Liu, Yu Huan 2 ;
作者机构： 1.Jiangxi Acad Agr Sci, Inst Agr Appl Microbiol, Nanchang 330200, Peoples R China
2.Sun Yat Sen Univ, Sch Life Sci, Guangzhou 510275, Guangdong, Peoples R China
关键词： Gene;Metagenomic library;Feruloyl esterase;Tannase
期刊名称：JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC （ 影响因子：2.269； 五年影响因子：2.328 ）
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年卷期：
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收录情况： SCI
摘要： A gene (tan410) encoding a feruloyl esterase was isolated by screening a cotton soil metagenomic library. Sequence analysis revealed that tan410 encodes a protein of 520 amino acids with a predicted molecular weight of 55 kDa. The gene was further expressed in Escherichia coli BL21 (DE3) using a pET expression system. The recombinant enzyme was purified and characterized. Its optimum temperature and pH were 35 °C and 7.0, respectively. Tan410 activity was enhanced by the addition of Mn~(2+), Mg~(2+), NH~(4+) and Ni~(2+). Besides ethyl ferulate, methyl caffeate, and methyl p-coumarate, Tan410 can also hydrolyze methyl gallate, tannic acid, epicatechin gallate, and epigallocatechin gallate which makes Tan410 an interesting enzyme for biotechnological applications.
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