Determination of Heparin in Plasma by HPLC Coupled with Resonance Light Scattering Detection
文献类型： 外文期刊
作者： Liao, Qie Gen 1 ; Li, Wei Hong 1 ; Luo, Lin Guang 1 ;
作者机构： 1.Jiangxi Acad Agr Sci, Key Lab Qual & Safety Agr Prod, Agr Prod Qual Safety & Stand Inst, Nanchang 330200, Peoples R China
关键词： High performance liquid chromatography;Resonance light scattering;Heparin
期刊名称：CHROMATOGRAPHIA （ 影响因子：2.044； 五年影响因子：1.725 ）
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收录情况： SCI
摘要： High performance liquid chromatography coupled with resonance light scattering detection was developed for separation and determination of heparin in plasma. A good chromatographic separation was achieved using an aminex HPX-87H column (300 mm × 7.8 mm, 9 μm) and a mobile phase of 5.0 mmol L~(-1) H2SO4 at the flow rate of 0.5 mL min~(-1). The enhanced resonance light scattering signals were derived from a large aggregate formation between heparin and cetyltrimethylammonium bromide used as the molecular recognition probe. The parameters of the post-column reaction (pH, concentration and flow rate of the reagent, length of the reaction coil, and temperature) were optimized. The limit of detection for heparin in plasma was 0.2 mg L~(-1). The method has been applied to the determination of heparin in dialysis patient plasmas.
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