Aptamer based fluorometric determination of kanamycin using double-stranded DNA and carbon nanotubes
文献类型： 外文期刊
作者： Liao, Qie Gen 1 ; Wei, Ben Hua 1 ; Luo, Lin Guang 1 ;
作者机构： 1.Jiangxi Acad Agr Sci, Lab Qual & Safety Risk Assessment Livestock & Pou, Agr Prod Qual Safety & Stand Inst, Minist Agr, Nanchang 330200, Jiangxi, Peoples R China
关键词： Kanamycin;Aptamer;Carbon nanotubes;Fluorescence Quenching;Milk analysis;Antibiotic
期刊名称：MICROCHIMICA ACTA （ 影响因子：5.833； 五年影响因子：5.357 ）
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收录情况： SCI
摘要： The authors describe a fluorometric assay for the antibiotic kanamycin that is making use of a fluorescein-labeled aptamer against kanamycin and carbon nanotubes (CNTs). It is found that CNTs do not quench the fluorescence of the duplex formed between FAM-labeled aptamer and its complementary DNA (cDNA). However, if kanamycin is added, it will bind to the aptamer, and this leads to dehybridization of the duplex upon which the fluorescence of the label is quenched by the CNTs present in solution. The method has an analytical range that extends from 1.0 to 50 nmol L-1 of kanamycin, and the detection limit is 0.4 nmol L-1. It was successfully employed to the detection of kanamycin in (spiked) milk samples and gave good recoveries.
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