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Large-scale identification of differentially expressed genes during pupa development reveals solute carrier gene is essential for pupal pigmentation in Chilo suppressalis

文献类型： 外文期刊

作者： Sun, Yang 1 ; Huang, Shuijin 2 ; Wang, Shuping 3 ; Guo, Dianhao 1 ; Ge, Chang 1 ; Xiao, Huamei 1 ; Jie, Wencai 1 ; Yang, 1 ;

作者机构： 1.Nanjing Agr Univ, Dept Entomol, Nanjing 210095, Jiangsu, Peoples R China

2.Jiangxi Acad Agr Sci, Inst Plant Protect, Nanchang 330200, Jiangxi, Peoples R China

3.Shanghai Entry Exit Inspect & Quarantine Bur, Tech Ctr Anim Plant & Food Inspect & Quarantine, Shanghai 200135, Peoples R China

4.Zhejiang Univ, Inst Insect Sci, Key Lab Agr Entomol, Minist Agr, 866 Yuhangtang Rd, Hangzhou 310058, Zhejiang, Peoples R China

关键词： Chilo suppressalis;Pupa development;Microarray;Gene expression profile;RNA interference;Solute carrier genes

期刊名称：JOURNAL OF INSECT PHYSIOLOGY （ 影响因子：2.354； 五年影响因子：3.045 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： Insects undergo metamorphosis, involving an abrupt change in body structure through cell growth and differentiation. Rice stem stripped borer (SSB), Chilo suppressalis, is one of the most destructive rice pests. However, little is known about the regulation mechanism of metamorphosis development in this notorious insect pest. Here, we studied the expression of 22,197 SSB genes at seven time points during pupa development with a customized microarray, identifying 622 differentially expressed genes (DEG) during pupa development. Gene ontology (GO) analysis of these DEGs indicated that the genes related to substance metabolism were highly expressed in the early pupa, which participate in the physiological processes of larval tissue disintegration at these stages. In comparison, highly expressed genes in the late pupal stages were mainly associated with substance biosynthesis, consistent with adult organ formation at these stages. There were 27 solute carrier (SLC) genes that were highly expressed during pupa development. We knocked down SLC22A3 at the prepupal stage, demonstrating that silencing SLC22A3 induced a deficiency in pupa stiffness and pigmentation. The RNAi-treated individuals had white and soft pupa, suggesting that this gene has an essential role in pupal development. (C) 2016 Elsevier Ltd. All rights reserved.

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