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Multiple miRNAs jointly regulate the biosynthesis of ecdysteroid in the holometabolous insects, Chilo suppressalis

文献类型： 外文期刊

作者： He, Kang 1 ; Sun, Yang 2 ; Xiao, Huamei 4 ; Ge, Chang 2 ; Li, Fei 1 ; Han, Zhaojun 2 ;

作者机构： 1.Zhejiang Univ, Minist Agr, Key Lab Mol Biol Crop Pathogens & Insects, Inst Insect Sci Coll Agr & Biotechnol, Hangzhou 310058, Zhejiang, Peoples R China

2.Nanjing Agr Univ, Dept Entomol, Coll Plant Protect, Nanjing 210095, Jiangsu, Peoples R China

3.Jiangxi Acad Agr Sci, Inst Plant Protect, Nanchang 330200, Jiangxi, Peoples R China

4.Yichun Univ, Coll Life Sci & Resource Environm, Yichun 336000, Peoples R China

关键词： insect;miRNA;metamorphosis;development;ecdysteroid

期刊名称：RNA （ 影响因子：4.942； 五年影响因子：4.921 ）

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收录情况： SCI

摘要： The accurate rise and fall of active hormones is important for insect development. The ecdysteroids must be cleared in a timely manner. However, the mechanism of suppressing the ecdysteroid biosynthesis at the right time remains unclear. Here, we sequenced a small RNA library of Chilo suppressalis and identified 300 miRNAs in this notorious rice insect pest. Microarray analysis yielded 54 differentially expressed miRNAs during metamorphosis development. Target prediction and in vitro dualluciferase assays confirmed that seven miRNAs (two conserved and five novel miRNAs) jointly targeted three Halloween genes in the ecdysteroid biosynthesis pathway. Overexpression of these seven miRNAs reduced the titer of 20-hydroxyecdysone (20E), induced mortality, and retarded development, which could be rescued by treatment with 20E. Comparative analysis indicated that the miRNA regulation of metamorphosis development is a conserved process but that the miRNAs involved are highly divergent. In all, we present evidence that both conserved and lineage-specific miRNAs have crucial roles in regulating development in insects by controlling ecdysteroid biosynthesis, which is important for ensuring developmental convergence and evolutionary diversity.

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