OsCIPK2 mediated rice root microorganisms and metabolites to improve plant nitrogen uptake
文献类型： 外文期刊
作者： Chen, Mengying 1 ; Feng, Shizhong 2 ; Lv, He 1 ; Wang, Zewen 1 ; Zeng, Yuhang 1 ; Shao, Caihong 4 ; Lin, Wenxiong 1 ; Zhang, Zhixing 1 ;
作者机构： 1.Fujian Agr & Forestry Univ, Coll JunCao Sci & Ecol, Fuzhou 350002, Fujian, Peoples R China
2.Fujian Agr & Forestry Univ, Key Lab Crop Ecol & Mol Physiol, Fuzhou 350002, Fujian, Peoples R China
3.Fujian Agr & Forestry Univ, Fujian Prov Key Lab Agroecol Proc & Safety Monitor, Fuzhou 350002, Peoples R China
4.Jiangxi Acad Agr Sci, Soil & Fertilizer & Resources & Environm Inst, Nanchang 330200, Peoples R China
关键词： OsCIPK2; Nitrogen uptake; Rhizosphere soil; Root microbiome; Metabolites; Synthetic microbial communities
期刊名称：BMC PLANT BIOLOGY （ 影响因子：5.3； 五年影响因子：5.9 ）
ISSN： 1471-2229
年卷期： 2024 年 24 卷 1 期
页码：
收录情况： SCI
摘要： Crop roots are colonized by large numbers of microorganisms, collectively known as the root-microbiome, which modulate plant growth, development and contribute to elemental nutrient uptake. In conditions of nitrogen limitation, the over-expressed Calcineurin B-like interacting protein kinase 2 (OsCIPK2) gene with root-specific promoter (RC) has been shown to enhance growth and nitrogen uptake in rice. Analysis of root-associated bacteria through high-throughput sequencing revealed that OsCIPK2 has a significant impact on the diversity of the root microbial community under low nitrogen stress. The quantification of nifH gene expression demonstrated a significant enhancement in nitrogen-fixing capabilities in the roots of RC transgenetic rice. Synthetic microbial communities (SynCom) consisting of six nitrogen-fixing bacterial strains were observed to be enriched in the roots of RC, leading to a substantial improvement in rice growth and nitrogen uptake in nitrogen-deficient soils. Forty and twenty-three metabolites exhibiting differential abundance were identified in the roots and rhizosphere soils of RC transgenic rice compared to wild-type (WT) rice. These findings suggest that OSCIPK2 plays a role in restructuring the microbial community in the roots through the regulation of metabolite synthesis and secretion. Further experiments involving the exogenous addition of citric acid revealed that an optimal concentration of this compound facilitated the growth of nitrogen-fixing bacteria and substantially augmented their population in the soil, highlighting the importance of citric acid in promoting nitrogen fixation under conditions of low nitrogen availability. These findings suggest that OsCIPK2 plays a role in enhancing nitrogen uptake by rice plants from the soil by influencing the assembly of root microbial communities, thereby offering valuable insights for enhancing nitrogen utilization in rice cultivation.
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