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Accumulation of the RNA polymerase subunit RpoB depends on RNA editing by OsPPR16 and affects chloroplast development during early leaf development in rice

文献类型： 外文期刊

作者： Huang, Weifeng 1 ; Zhang, Yang 1 ; Shen, Liqiang 2 ; Fang, Qian 1 ; Liu, Qun 1 ; Gong, Chenbo 1 ; Zhang, Chen 1 ; Zhou, Y 1 ;

作者机构： 1.Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan 430070, Peoples R China

2.Chinese Acad Sci, Shanghai Inst Plant Physiol & Ecol, CAS Ctr Excellence Mol Plant Sci, Key Lab Synthet Biol, Shanghai 200032, Peoples R China

3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China

4.Jiangxi Agr Univ, Coll Biosci & Bioengn, Nanchang 330045, Jiangxi, Peoples R China

5.Jiangxi Acad Agr Sci, Rice Res Inst, Key Lab Rice Physiol & Genet Jiangxi Prov, Nanchang Subctr Rice Natl Engn Lab, Nanchang 330200, Jiangxi, Peoples R China

6.Max Planck Inst Mol Pflanzenphysiol, Muhlenberg 1, D-14476 Potsdam, Germany

关键词： Chl biosynthesis; chloroplast development; plastid; RNA editing; RNA polymerase; transcription

期刊名称：NEW PHYTOLOGIST （ 影响因子：10.151； 五年影响因子：10.475 ）

ISSN： 0028-646X

年卷期： 2020 年 228 卷 4 期

页码：

收录情况： SCI

摘要： Plastid-encoded genes are coordinately transcribed by the nucleus-encoded RNA polymerase (NEP) and the plastid-encoded RNA polymerase (PEP). Resulting primary transcripts are frequently subject to RNA editing by cytidine-to-uridine conversions at specific sites. The physiological role of many editing events is largely unknown. Here, we have used the CRISPR/Cas9 technique in rice to knock out a member of the PLS-DYW subfamily of pentatricopeptide repeat (PPR) proteins. We found that OsPPR16 is responsible for a single editing event at position 545 in the chloroplastrpoBmessenger RNA (mRNA), resulting in an amino acid change from serine to leucine in the beta-subunit of the PEP. In striking contrast to loss-of-function mutations of the putative orthologue inArabidopsis, which were reported to have no visible phenotype, knockout ofOsPPR16leads to impaired accumulation of RpoB, reduced expression of PEP-dependent genes, and a pale phenotype during early plant development. Thus, by editing therpoBmRNA,OsPPR16is required for faithful plastid transcription, which in turn is required for Chl synthesis and efficient chloroplast development. Our results provide new insights into the interconnection of the finely tuned regulatory mechanisms that operate at the transcriptional and post-transcriptional levels of plastid gene expression.

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