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Escherichia coli heat-labile enterotoxin B subunit as an adjuvant of mucosal immune combined with GCRV-II VP6 triggers innate immunity and enhances adaptive immune responses following oral vaccination of grass carp (Ctenopharyngodon idella)

文献类型： 外文期刊

作者： Yin, Jiyuan 1 ; Wu, Huiliang 1 ; Li, Wei 1 ; Wang, Yingying 1 ; Li, Yingying 1 ; Mo, Xubing 1 ; Li, Siming 2 ; Ren, Yan 1 ; Pan, Houjun 1 ; Jiang, Peng 1 ; Wang, Qing 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Pearl River Fisheries Inst, Key Lab Fishery Drug Dev, Minist Agr & Rural Affairs,Key Lab Aquat Anim Immu, Guangzhou 510380, Guangdong, Peoples R China

2.Jiangxi Acad Agr Sci, Inst Qual & Safety & Stand Agr Prod Res, Nanlian Rd 602, Nanchang 330200, Peoples R China

关键词： GCRV; Oral vaccine; LTB; Lactococcus lactis; Mucosal adjuvant

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：3.9； 五年影响因子：4.2 ）

ISSN： 1050-4648

年卷期： 2024 年 154 卷

页码：

收录情况： SCI

摘要： The grass carp reovirus (GCRV) is the most major pathogen that has threatened the grass carp (Ctenopharyngodon idella) industry of China for years. Though the oral vaccine has many advantages, the current vaccines still do not provide complete protection. Therefor the exploration of new preventive strategies is urgently needed. In this study, heat-labile enterotoxin B subunit of Escherichia coli (LTB) was combined with VP6 from GCRV type II (GCRV-II) via Lactococcus lactis expression system to form a potent oral vaccine and determines if fusion of LTB to the protective vaccine antigen can enhance protection in the fish. The expression of recombinant protein was confirmed by Western-blotting and enzyme-linked immunosorbent assay. The rare minnow was set as the model for the evaluation of the experiment administrated orally. The immune response including the antibody titer and the immune-related gene expression, and the protective efficacy which included the virus loaded and the relative protection, were thoroughly investigated after the trial. The results indicated that LTB can significantly elicit a higher neutralizing antibody responses and enhanced T-cell priming, activities and proliferation in mononuclear cells from intestine, spleen and kidney tissues when compared to the VP6 vaccine alone. Moreover, the combined adjuvant can significantly up-regulate type I interferon signaling in different immune organs, especially the mucosa associated lymphoidtissue which could not be induced by VP6 along, result in the contribution of the improvement in adaptive immune responses of the fish. In addition, challenge study showed that LTB combined VP6 could greatly improve the relative percent survival of the fish during the virus infection. These results highlight that LTB has the potential value to be a mucosal adjuvant of the fish, approaching for improving the efficacy of vaccination against GCRV-II, which does elicit both non-specific and specific immune responses.

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