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Functional role of a novel zinc finger protein, AoZFA, in growth and kojic acid synthesis in Aspergillus oryzae

文献类型： 外文期刊

作者： Chen, Ziming 1 ; Chen, Tianming 1 ; Zhang, Huanxin 2 ; Li, Yuzhen 1 ; Fan, Junxia 1 ; Yao, Lihua 1 ; Zeng, Bin 1 ; Zhang, Zhe 1 ;

作者机构： 1.Jiangxi Sci & Technol Normal Univ, Coll Life Sci, Jiangxi Key Lab Bioproc Engn, Nanchang, Peoples R China

2.Jiangxi Acad Agr Sci, Inst Hort Sci, Nanchang, Peoples R China

3.Shenzhen Technol Univ, Coll Pharm, Shenzhen, Peoples R China

关键词： kojic acid; zinc finger protein; growth; Aspergillus oryzae; secondary metabolite

期刊名称：APPLIED AND ENVIRONMENTAL MICROBIOLOGY （ 影响因子：4.4； 五年影响因子：5.0 ）

ISSN： 0099-2240

年卷期： 2023 年

页码：

收录情况： SCI

摘要： Kojic acid (KA) is a valuable secondary metabolite that is regulated by zinc finger proteins in Aspergillus oryzae. However, only two such proteins have been characterized to function in kojic acid production of A. oryzae to date. In this study, we identified a novel zinc finger protein, AoZFA, required for kojic acid biosynthesis in A. oryzae. Our results showed that disruption of AozfA led to increased expression of kojA and kojR involved in kojic acid synthesis, resulting in enhanced kojic acid production, while overexpression of AozfA had the opposite effect. Furthermore, deletion of kojR in the AozfA disruption strain abolished kojic acid production, whereas overexpression of kojR enhanced it, indicating that AoZFA regulates kojic acid production by affecting kojR. Transcriptional activation assay revealed that AoZFA is a transcriptional activator. Interestingly, when kojR was overexpressed in the AozfA overexpression strain, the production of kojic acid failed to be rescued, suggesting that AozfA plays a distinct role from kojR in kojic acid biosynthesis. Moreover, we found that AozfA was highly induced by zinc during early growth stages, and its overexpression inhibited the growth promoted by zinc, whereas its deletion had no effect, suggesting that AoZFA is non-essential but has a role in the response of A. oryzae to zinc. Overall, these findings provide new insights into the roles of zinc finger proteins in the growth and kojic acid production of A. oryzae.

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