文献类型： 外文期刊

作者： Xu, J. G. ² ; Xie, M. G. ⁵ ; Zou, S. Y. ¹ ; Liu, X. F. ¹ ; Li, X. H. ¹ ; Xie, J. F. ⁶ ; Zhang, X. Q. ¹ ;

作者机构： 1.South China Agr Univ, Coll Anim Sci, Guangzhou, Guangdong, Peoples R China

2.South China Agr Univ, Guangdong Prov Key Lab Agroanim Genom & Mol Breed, Guangzhou, Guangdong, Peoples R China

3.South China Agr Univ, Minist Agr, Key Lab Chicken Genet Breeding & Reprod, Guangzhou, Guangdong, Peoples R China

4.Guangzhou Quancheng Biotechnol Co Ltd, Guangzhou, Guangdong, Peoples R China

5.Jiangxi Acad Agr Sci, Inst Anim Husb & Vet, Nanchang, Peoples R China

6.Jiangxi Acad Agr Sci, Inst Anim Husb & Vet,

关键词： Gray dilution phenotype;MC1R;EDN3;MLPH;RAB27A;MYO5A

期刊名称：GENETICS AND MOLECULAR RESEARCH （ 影响因子：0.764； 五年影响因子：0.912 ）

ISSN： 1676-5680

年卷期： 2016 年 15 卷 2 期

页码：

收录情况： SCI

摘要： The Anyi tile-like gray chicken is a Chinese indigenous breed with a gray dilution phenotype, having gray feathers, comb, skin, shanks, and beak, which is valuable for genetic research on pigmentation. However, the genetic basis of the gray dilution phenotype remains unknown. The objective of this study was to investigate the genetic basis of the gray dilution phenotype in the Anyi tile-like gray chicken. We found that all Anyi tile-like gray chickens tested in this study carried at least one E allele, which is responsible for the appearance of black feathers, and some of them carried the FM allele, which is responsible for the black skin phenotype. A single nucleotide polymorphism (C.1909A>G) was identified within the melanophilin (MLPH) gene and was significantly associated with the gray dilution phenotype. Our findings suggest that the E and FM alleles act together to cause the development of the "five-black" phenotype (black feather, comb, skin, shank, and beak), whereas the MLPH mutation results in defective melanosome transport, leading to the development of the "five-gray" phenotype.