Rapid diagnosis of different goose astrovirus genotypes with Taqman-based duplex real-time quantitative PCR
文献类型： 外文期刊
作者： Li, Haiqin 1 ; Kang, Zhaofeng 2 ; Wan, Chunhe 2 ; Zhang, Fanfan 2 ; Tan, Meifang 2 ; Zeng, Yanbing 2 ; Wu, Chengcheng 2 ; Huang, Yu 3 ; Su, Qi 4 ; Guo, Xiaoquan 1 ;
作者机构： 1.Jiangxi Agr Univ, Coll Anim Sci & Technol, Jiangxi Prov Key Lab Anim Hlth, Nanchang, Peoples R China
2.Jiangxi Acad Agr Sci, Inst Anim Husb & Vet Med, Nanchang 330200, Jiangxi, Peoples R China
3.Fujian Acad Agr Sci, Inst Anim Husb & Vet Med, Fuzhou 350013, Fujian, Peoples R China
4.Shandong Agr Univ, Coll Vet Med, Tai An 271000, Shandong, Peoples R China
关键词： Key words; goose astrovirus; genotype; diagnosis; Taqman; genome
期刊名称：POULTRY SCIENCE （ 影响因子：4.4； 五年影响因子：4.4 ）
ISSN： 0032-5791
年卷期： 2023 年 102 卷 7 期
页码：
收录情况： SCI
摘要： The epidemic of goose astrovirus (GoAstV) caused huge losses to the poultry industry. Epidemiological studies in China revealed 2 circulating genotypes of GoAstV, but there is a lack of differential diagnosis tools. By analyzing all published genomes of GoAstV, this study designed specific PCR primers and Taqman probes to recognize different genotypes of GoAstV. After optimization and verification, this study developed a Taqman-based real-time quantitative PCR method that is capable of differential diagnosis. The established qPCR exhibited detection limitations of 100 copies/mL or 10 copies/mL, respectively, for GoAstV genotype 1 and genotype 2, and showed no false positive for other common avian viruses. This method was then used to analyze 72 samples collected from different regions in Jiangxi, and the results were verified by genome sequencing and phylogenetic analysis. These results revealed a complex coinfection of GoAstV different geno-types in China, highlighting the importance of long-term focus on the prevalence and genome evolution of GoAstV.
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