Rapid diagnosis of different goose astrovirus genotypes with Taqman-based duplex real-time quantitative PCR
文献类型： 外文期刊
作者： Li, Haiqin 1 ; Kang, Zhaofeng 2 ; Wan, Chunhe 2 ; Zhang, Fanfan 2 ; Tan, Meifang 2 ; Zeng, Yanbing 2 ; Wu, Chengcheng 2 ; Huang, Yu 3 ; Su, Qi 4 ; Guo, Xiaoquan 1 ;
作者机构： 1.Jiangxi Agr Univ, Coll Anim Sci & Technol, Jiangxi Prov Key Lab Anim Hlth, Nanchang, Peoples R China
2.Jiangxi Acad Agr Sci, Inst Anim Husb & Vet Med, Nanchang 330200, Jiangxi, Peoples R China
3.Fujian Acad Agr Sci, Inst Anim Husb & Vet Med, Fuzhou 350013, Fujian, Peoples R China
4.Shandong Agr Univ, Coll Vet Med, Tai An 271000, Shandong, Peoples R China
关键词： Key words; goose astrovirus; genotype; diagnosis; Taqman; genome
期刊名称：POULTRY SCIENCE （ 影响因子：4.4； 五年影响因子：4.4 ）
ISSN： 0032-5791
年卷期： 2023 年 102 卷 7 期
页码：
收录情况： SCI
摘要： The epidemic of goose astrovirus (GoAstV) caused huge losses to the poultry industry. Epidemiological studies in China revealed 2 circulating genotypes of GoAstV, but there is a lack of differential diagnosis tools. By analyzing all published genomes of GoAstV, this study designed specific PCR primers and Taqman probes to recognize different genotypes of GoAstV. After optimization and verification, this study developed a Taqman-based real-time quantitative PCR method that is capable of differential diagnosis. The established qPCR exhibited detection limitations of 100 copies/mL or 10 copies/mL, respectively, for GoAstV genotype 1 and genotype 2, and showed no false positive for other common avian viruses. This method was then used to analyze 72 samples collected from different regions in Jiangxi, and the results were verified by genome sequencing and phylogenetic analysis. These results revealed a complex coinfection of GoAstV different geno-types in China, highlighting the importance of long-term focus on the prevalence and genome evolution of GoAstV.
- 相关文献
作者其他论文 更多>>
-
Prevalence and phylogenetic analysis of Gyrovirus galga 1 in southern China from 2020 to 2022
作者：Zhang, Fanfan;Yang, Qun;Wu, Chengcheng;Kang, Zhaofeng;Xie, Quan;Luo, Yangyang;Wan, Peiwei;Chen, Jiajia;Tu, Lingyun
关键词：Gyrovirus galga 1; prevalence; complete genome; genetic divergence; southern China
-
Detection and genetic diversity of subgroup K avian leukosis virus in local chicken breeds in Jiangxi from 2021 to 2023
作者：Zhang, Fanfan;Li, Haiqin;Lin, Cui;Wei, Yue;Zhang, Weihong;Wu, Yanping;Kang, Zhaofeng
关键词：avian leukosis virus subgroup K; prevalence; genetic diversity; gp85 gene; avian leukosis virus
-
Integrating miRNA and full-length transcriptome profiling to elucidate the mechanism of muscle growth in Muscovy ducks reveals key roles for miR-301a-3p/ANKRD1
作者：Huang, Jiangnan;Xiong, Xiaolan;Zhang, Weihong;Chen, Xiaolian;Wei, Yue;Li, Haiqin;Xie, Jinfang;Wei, Qipeng;Zhou, Quanyong
关键词：Muscovy ducks; Muscle growth; microRNA sequencing; Full-length transcriptome; miR-301a-3p; ANKRD1
-
Epidemiological monitoring and genetic variation analysis of pathogens associated with porcine viral diarrhea in southern China from 2021 to 2023
作者：Zhang, Fanfan;Lin, Cui;Tan, Meifang;Xiong, Ligen;Ji, Huayuan;Luo, Yangyang;Wan, Peiwei;Xie, Baobao
关键词：porcine diarrhea; prevalence; porcine epidemic diarrhea virus; porcine deltacoronavirus; swine acute diarrhea syndrome coronavirus; porcine rotavirus
-
Research Note: Pathogenetic characteristics of avian encephalomyelitis virus in Guangdong and Jiangxi Provinces, China
作者：Zhang, Fanfan;Wei, Qipeng;Xiong, Ligen;Tan, Jia;Wu, Chengcheng;Li, Na;Kang, Zhaofeng;Luo, Yangyang;Xie, Quan
关键词：avian encephalomyelitis virus; surveillance; epidemiological characteristic; phylogenetic analysis
-
Research Note: Transcriptome analysis of skeletal muscles of black-boned chickens, including 2 types (wild and mutated) of Taihe black-boned silky fowl and 1 type (wild) of Yugan black-boned chicken
作者：Huang, Cong;Wei, Yue;Kang, Zhaofeng;Zhang, Weihong;Wu, Yanping
关键词：Taihe black-boned silky fowl; transcriptome; melanin; skeletal muscle; GSEA
-
Rapid detection of goose astrovirus genotypes 2 using real-time reverse transcription recombinase polymerase amplification
作者：Li, Haiqin;Tan, Meifang;Zhang, Fanfan;Zeng, Yanbing;Huang, Jiangnan;Wu, Chengcheng;Kang, Zhaofeng;Li, Haiqin;Guo, Xiaoqiao;Zhu, Yujun;Zhu, Yujun;Wan, Chunhe;Huang, Yu;Wang, Zhangzhang;Liu, Lei
关键词：Goose astrovirus genotypes 2; Real-time; Reverse transcription recombinase polymerase amplification; Point-of-care diagnosis
