文献类型： 外文期刊

作者： Luo Xiang-dong ¹ ; Liu Jian ¹ ; Zhao Jun ¹ ; Dai Liang-fang ¹ ; Chen Ya-ling ¹ ; Zhang Ling ¹ ; Zhang Fan-tao ¹ ; Hu Biao ¹ ;

作者机构： 1.Jiangxi Normal Univ, Coll Life Sci, Nanchang 330022, Jiangxi, Peoples R China

2.Jiangxi Acad Agr Sci, Rice Res Inst, Nanchang 330200, Jiangxi, Peoples R China

关键词： common wild rice;cold tolerance;QTL-seq;single nucleotide polymorphisms (SNP)

期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：2.848； 五年影响因子：2.979 ）

ISSN： 2095-3119

年卷期： 2018 年 17 卷 2 期

页码：

收录情况： SCI

摘要： Cold stress is a major problem in rice production. To rapidly identify genes for cold tolerance in Dongxiang wild rice (DWR, Oryza rufipogon Griff.), sequencing-based bulked segregant analysis of QTL-seq method was used to resequence the extremely resistant (R) and susceptible (S) bulks of a backcross inbred lines (BILs) population (derived from Oryza sativaxO. rufipogon) and their parents. Single nucleotide polymorphisms (SNP)-index graphs and corresponding Delta(SNP-index) graphs (at 99 and 95% confidence levels) for R-and S-bulks detected a total of 2 609 candidate SNPs, including 58 candidate cold-tolerance genes. Quantitative real-time PCR analysis revealed that 5 out of the 58 candidate genes had significant differences in expression between O. sativa and O. rufipogon. Structural variation and functional annotations of the 5 candidate genes were also analyzed, and allowed us to identify 2 insertion-deletion (InDel) markers (12-7 and 12-16) that were linked with candidate genes on chromosome 12 in DWR. These results are helpful for cloning and using cold tolerance genes from common wild rice in cultivated rice.