您好，欢迎访问江西省农业科学院 机构知识库！

Isolation and Characterization of DkPK Genes Associated with Natural Deastringency in C-PCNA Persimmon

文献类型： 外文期刊

作者： Guan, Changfei 1 ; Chen, Wenxing 1 ; Mo, Rongli 1 ; Du, Xiaoyun 2 ; Zhang, Qinglin 1 ; Luo, Zhengrong 1 ;

作者机构： 1.Huazhong Agr Univ, Key Lab Hort Plant Biol, Wuhan, Peoples R China

2.Jiangxi Acad Agr Sci, Inst Hort Sci, Nanchang, Peoples R China

3.Hubei Collaborat Innovat Ctr Characterist Resourc, Huanggang, Peoples R China

关键词： persimmon;PAs;DkPK;qRT-PCR;transient over-expression;deastringency

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：5.753； 五年影响因子：6.612 ）

ISSN： 1664-462X

年卷期： 2016 年 7 卷

页码：

收录情况： SCI

摘要： Chinese pollination-constant non-astringent (C-PCNA) persimmon (Diospyros kaki Thunb.) is considered to be an important germplasm resource for the breeding of PCNA cultivars, though its molecular mechanisms of astringency removal remain to be elucidated. Previously, we showed that the abundance of pyruvate kinase gene transcripts increased rapidly during astringency removal in C-PCNA persimmon fruit. Here, we report the full-length coding sequences of six novel DkPK genes from C-PCNA persimmon fruit isolated based on a complementary DNA (cDNA) library and transcriptome data. The expression patterns of these six DkPK genes and correlations with the soluble proanthocyanidin (PA) content were analyzed during various fruit development stages in different types of persimmon, with DkPK1 showing an expression pattern during the last stage in C-PCNA persimmon that was positively correlated with a decrease in soluble PAs. Phylogenetic analysis revealed that DkPK1 belongs to cytosolic-1 subgroup, and subcellular localization analysis confirmed that DkPK1 is located in the cytosol. Notably, tissue expression profiling revealed ubiquitous DkPK1 expression in different persimmon organs, with the highest expression in seeds. Furthermore, transient over-expression of DkPK1 in persimmon leaves resulted in a significant decrease in the content of soluble PAs but a significant increase in the transcript levels of pyruvate decarboxylase genes (DkPDC1, -3, -4, -5), which catalyze the conversion of pyruvate to acetaldehyde. Thus, we propose that an acetaldehyde based coagulation effect reduces the content of soluble PAs. Taken together, our results suggest that DkPK1 might be involved in the natural removal of astringency at the last developmental stage in C-PCNA persimmon. This is the first report to identify several novel full-length DkPK genes as well as their potential roles in the natural loss of astringency in C-PCNA persimmon.

  • 相关文献

[1]SSAP analysis reveals candidate genes associated with deastringency in persimmon (Diospyros kaki Thunb.) treated with 40 degrees C water. Guan, Changfei,Chen, Li,Chen, Wenxing,Mo, Rongli,Zhang, Qinglin,Liu, Jihong,Luo, Zhengrong,Du, Xiaoyun. 2015

[2]Genetic Diversity of Persimmon Landraces as Assessed by Morphological Traits and IRAP Molecular Markers in Jiangxi Province. Du, X.,Zen, M.,Wang, Y.,Xiong, Y.,Huang, J.,Le, M.,Pei, X.,Luo, Z..

[3]Transferability of retrotransposon primers derived from Persimmon (Diospyros kaki Thunb.) across other plant species. Du, X. Y.,Wang, Y. B.,Du, X. Y.,Hu, Q. N.,Zhang, Q. L.,Luo, Z. R.. 2013

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：江西省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台