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SSAP analysis reveals candidate genes associated with deastringency in persimmon (Diospyros kaki Thunb.) treated with 40 degrees C water

文献类型： 外文期刊

作者： Guan, Changfei 1 ; Chen, Li 1 ; Chen, Wenxing 1 ; Mo, Rongli 1 ; Zhang, Qinglin 1 ; Du, Xiaoyun 2 ; Liu, Jihong 1 ; Luo, 1 ;

作者机构： 1.Huazhong Agr Univ, Key Lab Hort Plant Biol MOE, Wuhan 430070, Peoples R China

2.Jiangxi Acad Agr Sci, Inst Hort Sci, Nanchang 330200, Peoples R China

关键词： Persimmon;40 degrees C water;Deastringency;SSAP;Transcript-derived fragments;qRT-PCR

期刊名称：TREE GENETICS & GENOMES （ 影响因子：2.297； 五年影响因子：2.547 ）

ISSN： 1614-2942

年卷期： 2015 年 11 卷 2 期

页码：

收录情况： SCI

摘要： Warm water treatment is a simple and effective deastringency method for the persimmon fruit. The astringency in the 'Eshi 1' persimmon, a Chinese pollination-constant and non-astringent (C-PCNA) cultivar, can be quickly removed in 40 degrees C water coupled with the activation of the retrotransposon DkRE1. In this study, transcriptional level changes in 'Eshi 1' persimmon treated with 40 degrees C water were studied with a sequence-specific amplification polymorphism (SSAP) approach to investigate the genes associated with deastringency. The complementary DNA (cDNA)-SSAP analysis revealed that 283 differentially expressed transcript-derived fragments (TDFs) potentially involved in 'Eshi 1' persimmon deastringency were successfully cloned and sequenced. In total, 116 differentially expressed TDFs were annotated as genes with known function, including four aldehyde metabolism genes and two pectin-related genes. Our data suggested that the tannin-pectin complex formation and the aldehyde-mediated coagulation effect contributed to the deastringency of the persimmon fruit treated with 40 degrees C water. Notably, three gene fragments, triosephosphate isomerase (TDF 175-3), pyruvate kinase (TDF 284-2), and neomenthol dehydrogenase (TDF 238-2), which are potentially involved in the 40 degrees C water coagulation effect were identified. To our knowledge, this report is the first to identify these gene fragments in the persimmon. The SSAP method was innovatively applied to gene discovery based on high-density polymorphisms. Expression analysis and transcriptome data confirmed the validity and accuracy of the cDNA-SSAP method for discovering new candidate genes. These successfully isolated genes could facilitate understanding of astringency removal in persimmon.

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[1]Isolation and Characterization of DkPK Genes Associated with Natural Deastringency in C-PCNA Persimmon. Guan, Changfei,Chen, Wenxing,Mo, Rongli,Zhang, Qinglin,Luo, Zhengrong,Du, Xiaoyun,Zhang, Qinglin,Luo, Zhengrong. 2016

[2]逆转座子分子标记及其在果树上的应用. 杜晓云,张青林,罗正荣. 2009

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[4]Transferability of retrotransposon primers derived from Persimmon (Diospyros kaki Thunb.) across other plant species. Du, X. Y.,Wang, Y. B.,Du, X. Y.,Hu, Q. N.,Zhang, Q. L.,Luo, Z. R.. 2013

[5]Genetic Diversity of Persimmon Landraces as Assessed by Morphological Traits and IRAP Molecular Markers in Jiangxi Province. Du, X.,Zen, M.,Wang, Y.,Xiong, Y.,Huang, J.,Le, M.,Pei, X.,Luo, Z..

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