Rapid diagnosis of duck Tembusu virus and goose astrovirus with TaqMan-based duplex real-time PCR
文献类型： 外文期刊
作者： Li, Haiqin 1 ; Wan, Chunhe 3 ; Wang, Zhangzhang 4 ; Tan, Jia 2 ; Tan, Meifang 2 ; Zeng, Yanbing 2 ; Huang, Jiangnan 2 ; Huang, Yu 3 ; Su, Qi 5 ; Kang, Zhaofeng 2 ; Guo, Xiaoquan 1 ;
作者机构： 1.Jiangxi Agr Univ, Coll Anim Sci & Technol, Jiangxi Prov Key Lab Anim Hlth, Nanchang, Peoples R China
2.Jiangxi Acad Agr Sci, Inst Anim Husb & Vet Med, Nanchang, Jiangxi, Peoples R China
3.Fujian Acad Agr Sci, Inst Anim Husb & Vet Med, Fuzhou, Fujian, Peoples R China
4.Xingguo Cty Agr Technol Extens Ctr, Ganzhou, Jiangxi, Peoples R China
5.Shandong Agr Univ, Coll Vet Med, Tai An, Shandong, Peoples R China
关键词： diagnosis; Tembusu virus; goose astrovirus; TaqMan; duplex qPCR
期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：5.2； 五年影响因子：6.2 ）
ISSN：
年卷期： 2023 年 14 卷
页码：
收录情况： SCI
摘要： The mixed infection of duck Tembusu virus (DTMUV) and goose astrovirus (GoAstV) is an important problem that endangers the goose industry. Although quantitative PCR has been widely used in monitoring these two viruses, there is no reliable method to detect them at the same time. In this study, by analyzing the published genomes of DTMUV and goose astrovirus genotype 2 (GoAstV-2) isolated in China, we found that both viruses have high conservation, showing 96.5 to 99.5% identities within different strains of DTMUV and GoAstV, respectively. Subsequently, PCR primers and TaqMan probes were designed to identify DTMUV and GoAstV-2, and different fluorescent reporters were given to two probes for differential diagnosis. Through the optimization and verification, this study finally developed a duplex TaqMan qPCR method that can simultaneously detect the above two viruses. The lower limits of detection were 100 copies/mu L and 10 copies/mu L for DTMUV and GoAstV-2 under optimal condition. The assay was also highly specific in detecting one or two viruses in various combinations in specimens, and provide tool for clinical diagnosis of mixed infections of viruses in goose.
- 相关文献
作者其他论文 更多>>
-
Goose astrovirus induces apoptosis and endoplasmic reticulum stress in gosling hepatocytes
作者：Lu, Zhihua;Li, Haiqin;Gao, Xiaona;Fu, Duanfeng;Huang, Haoyu;Huang, Cheng;Wu, Meiqin;Guo, Xiaoquan;Li, Haiqin
关键词：Goose astrovirus virus; Endoplasmic reticulum stress; Apoptosis; Gosling
-
LC/MS-based lipidomics and transcriptomics reveal lipid diversity and regulatory networks underlying intramuscular fat differences in Xingguo grey geese
作者：Zou, Mengyun;Zhang, Weihong;Xiong, Xiaolan;Rao, Linjie;Wei, Qipeng;Li, Haiqin;Zhang, Fanfan;Xie, Jinfang;Huang, Jiangnan;Wang, Zhangzhang
关键词：LC/MS-based lipidomics; Transcriptomics; Intramuscular fat; Xingguo grey goose; Breast muscle
-
Two opposite abilities of the infectious bronchitis virus helicase Nsp13: separating the duplex and promoting the annealing of single-stranded nucleic acid
作者：Wu, Chengcheng;Li, Haiqin;Huang, Cong;Zeng, Yanbing;Tao, Lihan
关键词：coronavirus; helicase; unwinding; annealing; replication mechanism
-
Immune-iron homeostasis deciphers resistance divergence to Riemerella anatipestifer in ducks
作者：Zou, Mengyun;Xiong, Xiaolan;Zhang, Weihong;Wei, Yue;Tang, Weiguo;Wei, Qipeng;Huang, Cong;Kang, Zhaofeng;Xie, Jinfang;Huang, Jiangnan
关键词：duck;
Riemerella anatipestifer ; disease resistance; immune response; iron metabolism -
Identification of a novel linear B-cell epitope recognized by a monoclonal antibody against the capsid protein of goose astrovirus genotypes 2
作者：Li, Haiqin;Huang, Haoyu;Lin, Cui;Zhang, Fanfan;Tan, Jia;Li, Na;Wu, Chengcheng;Huang, Jiangnan;Wei, Qipeng;Wang, Siyi;Zeng, Yanbing;Zhu, Yujun
关键词：Goose astrovirus genotypes 2; Cap protein; Monoclonal antibody; B-cell linear epitope
-
The Stalk and 1B Domains Are Required for Porcine Deltacoronavirus Helicase NSP13 to Separate the Double-Stranded Nucleic Acids, and the Deletion of the ZBD Impairs This Activity
作者：Wu, Chengcheng;Zhou, Quanyong;Zhang, Fanfan;Zeng, Yanbing;Tao, Lihan
关键词：porcine deltacoronavirus; helicase; domains; unwinding activity; ATPase activity
-
Effects and potential pathways of goose astrovirus infection on gosling hepatic lipid metabolism
作者：Yin, Chao;Shi, Yun;Lu, Zhihua;Gao, Xiaona;Hu, Guoliang;Guo, Xiaoquan;Li, Haiqin
关键词：astrovirus; gosling; infection; lipid metabolism; liver; pathway
