文献类型： 外文期刊

作者： Qin, Wan-Qi ¹ ; Fei, Dan ³ ; Zhou, Lei ¹ ; Guo, Yu-Jia ¹ ; An, Shun ¹ ; Gong, Ou-Hang ¹ ; Wu, Yun-Yang ¹ ; Liu, Jin-Feng ¹ ; Yang, Shi-Zhong ¹ ; Mu, Bo-Zhong ¹ ;

作者机构： 1.East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

2.East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China

3.Jiangxi Acad Agr Sci, Inst Qual Safety & Stand Agr Prod, Nanchang 330200, Jiangxi, Peoples R China

4.Daqing Huali Biotechnol Co Ltd, Daqing 163511, Heilongjiang, Peoples R China

5.Shanghai Collaborat Innovat Ctr Biomfg Technol, Shanghai 200237, Peoples R China

期刊名称：NEW JOURNAL OF CHEMISTRY （ 影响因子：3.3； 五年影响因子：3.2 ）

ISSN： 1144-0546

年卷期： 2023 年 47 卷 16 期

页码：

收录情况： SCI

摘要： A new member of the surfactin family of microbial lipopeptides has been isolated from the cell broth of Bacillus subtilis TD7 using a combination of organic solvent extraction and RP-HPLC, and its primary chemical structure was determined by ESI-MS, LC-MS/MS, GC-MS, H-1-NMR, C-13-NMR and amino acid analysis. The surfactin-C-17 consists of a beta-hydroxy heptadecanoic acid chain and a heptapeptide ring with the sequence of N-Glu-Leu-Leu-Val-Asp-Leu-Leu-C, which is a new member with the longest hydrophobic chain in the surfactin family produced by B. subtilis. Compared with the reported members of surfactins-C-11 similar to 16, surfactin-C-17 showed a very low critical micelle concentration (CMC) of 4.02 x 10(-6) mol L-1 in 10 mM PBS at 25 degrees C, and a very low surface tension of 28.98 mN m(-1) at CMC (gamma(CMC)); meanwhile, it inherited a strong antibacterial activity against the Gram-positive Micrococcus luteus with a MIC value of 60 mg L-1, and a higher hemolytic activity on sheep erythrocytes. This work concluded that increasing the hydrophobic chain length of a surfactin certainly contributed to decreasing its CMC and would facilitate the development of environmentally friendly biosurfactants and extend the domain of surfactin biosurfactants to new applications such as in pharmaceutical, bioremediation and detergent industries.