您好，欢迎访问江西省农业科学院 机构知识库！

Active microbial taxa preferentially assimilate inorganic nitrogen in acidic upland soils using a 15N-DNA-SIP approach

文献类型： 外文期刊

作者： Liu, Ran 1 ; Han, Cheng 1 ; Kang, Yu 3 ; Jiang, Yunbin 1 ; Deng, Huan 4 ; Liu, Kailou 5 ; Zhang, Jinbo 1 ; Zhong, Wenhui 1 ;

作者机构： 1.Nanjing Normal Univ, Sch Geog Sci, Jiangsu Prov Key Lab Mat Cycling & Pollut Control, Nanjing 210023, Peoples R China

2.Jiangsu Ctr Collaborat Innovat Geog Informat Resou, Nanjing 210023, Peoples R China

3.Enshi Tujia&Miao Autonomous Prefecture Acad Agr Sc, Enshi 445000, Peoples R China

4.Nanjing Normal Univ, Sch Environm, Nanjing 210023, Peoples R China

5.Jiangxi Inst Red Soil & Germplasm Resources, Nanchang 331717, Peoples R China

关键词： Inorganic nitrogen immobilization; Soil nitrogen retention; Organic nitrogen; Active microorganisms; 15 N isotope labeling

期刊名称：SOIL BIOLOGY & BIOCHEMISTRY （ 影响因子：9.7； 五年影响因子：10.2 ）

ISSN： 0038-0717

年卷期： 2024 年 188 卷

页码：

收录情况： SCI

摘要： Soil endogenous nitrogen is an essential source of crop nitrogen supply. Nitrogen assimilation is a vital transformation process that maintains nitrogen availability and promotes the mineralization of endogenous organic nitrogen in acidic soils. However, the dominant microbial populations that assimilate NH4+ and NO3- in acidic upland soils were unknown, and their response to long -term fertilization remained unclear. In this study, 15N- labeled NH4+ and NO3- were added to acidic upland soils characterized by long -term (32 years) differentiation in organic matter inputs. Glucose was added to facilitate nitrogen assimilation, and a nitrification inhibitor (dicyandiamide, DCD) was added to distinguish the contribution of NH4+ and NO3- assimilation. Results showed that soil inorganic N assimilation mainly occurred in the first week after inorganic N addition. Soil net inorganic N assimilation rates were 0.006-0.42 mg N kg -1 d-1 in the NH4+, NO3- and NH4++DCD treatments. The analysis of 15N -DNA -based stable isotope probing (SIP) found that the majority of inorganic N assimilation in our soils was conducted by a limited number of active microbial taxa, which accounted for 1.4%-2.0% of the entire bacterial community and 15.7%-28.8% of the fungal community. The dominant bacteria governing inorganic N assimilation in acidic upland soils included Bacillus, Burkholderia-Caballeronia-Paraburkholderia and Micrococcaceae. The primary fungi included Penicillium, Chaetomium, Aspergillus, and Fusarium. No obvious bias was detected in nitrogen assimilation rates and the dominant microbial taxa for NH4+-N and NO3- -N. Compared with unfertilized soil, long -term organic matter input decreased the dominating N assimilating microbial taxa. These findings demonstrate the feasibility of 15N -DNA -SIP technology in soil nitrogen cycling research and suggest the importance of active microbial taxa in acidic agricultural soil nitrogen retention and loss.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：江西省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台