文献类型： 外文期刊

作者： Jia, Y. ¹ ; Correa-Victoria, F. ² ; McClung, A. ² ; Zhu, L. ² ; Liu, G. ³ ; Wamishe, Y.; Xie, J.; Marchetti, M. A.; ¹ ;

作者机构： 1.USDA ARS, Dale Bumpers Natl Rice Res Ctr, Stuttgart, AR 72160 USA

2.USDA ARS, Dale Bumpers Natl Rice Res Ctr, Stuttgart, AR 72160 USA; Int Ctr Trop Agr, Cali AA 6713, Colombia; USDA ARS, Rice Res & Extens Ctr, Stuttgart, AR 72160 USA; Jiangxi Acad Agr Sci, Nanchang 330200, Peoples R China; USDA ARS, Beaumont, TX 77713 USA; Univ Arkansas, Fayetteville, AR 72701 USA

3.USDA ARS, Dale Bumpers Natl Rice Res Ctr, Stuttgart, AR 7

关键词： Agar;Economics;Greenhouses;Humidity;Agar;Economics;Greenhouses;Humidity;Inoculation;Pathogens;Plant breeding;Plant diseases;Seedlings;Sheath blight;Oryza sativa;Rhizoctonia solani;Solanum tuberosum

期刊名称：PLANT DISEASE （ 影响因子：4.438； 五年影响因子：4.7 ）

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收录情况： SCI

摘要： An accurate greenhouse screening method has not been developed previously to identify host response to sheath blight disease caused by Rhizoctonia solani Kuehn that causes significant economic losses in rice yield worldwide. The unavailability of a robust screening system in the greenhouse has made it difficult to quantify disease reactions to R. solani, and has hampered studies on the genetics of resistance and plant breeding efforts to improve resistance. In an effort to develop a standardized laboratory micro-chamber screening method to quantify resistance to R. solani in rice, five rice cultivars, representing a wide range of observed disease reactions under field conditions, were examined in a blind inoculation test at three locations (Arkansas, Texas, and Colombia). Rice seedlings were inoculated at the three- to four-leaf stage with potato dextrose agar plugs containing mycelium and then covered with a 2- or 3-liter transparent plastic bottle for maintaining high humidity after inoculation. Two cultivars, Jasmine 85 and Lemont, that consistently have shown the highest and lowest levels of resistance, respectively, in previous field and greenhouse studies, were used as standards. Concurrent field experiments in Arkansas and Texas also were performed to compare the greenhouse disease ratings with those observed under field conditions. Overall, the relative disease ratings of the seven test cultivars were consistent between test locations and with field evaluations. Thus, the micro-chamber screening method can be used as an effective approach to accurately quantify resistance to the sheath blight pathogen under controlled greenhouse conditions and should help expedite the selection process to improve resistance to this important pathogen.