Identification, Analysis, and Confirmation of Seed Storability-Related Loci in Dongxiang Wild Rice (Oryza rufipogon Griff.)
文献类型： 外文期刊
作者： Zhao, Minmin 1 ; Hu, Biaolin 2 ; Fan, Yuanwei 3 ; Ding, Gumu 1 ; Yang, Wanling 4 ; Chen, Yong 4 ; Chen, Yanhong 1 ; Xie, Jiankun 4 ; Zhang, Fantao 1 ;
作者机构： 1.Jiangxi Normal Univ, Coll Life Sci, Nanchang 330022, Jiangxi, Peoples R China
2.Jiangxi Acad Agr Sci, Rice Res Inst, Rice Natl Engn Lab, Nanchang 330022, Jiangxi, Peoples R China
3.Beijing Forestry Univ, Coll Biol Sci & Biotechnol, Beijing 100083, Peoples R China
4.Jiangxi Prov Key Lab Protect & Utilizat Subtrop P, Nanchang 330022, Jiangxi, Peoples R China
5.Washington Univ, Dept Biol, Campus Box 1137, St Louis, MO 63130 USA
6.Washington Univ, Ctr Engn Mechanobiol, Campus Box 1137, St Louis, MO 63130 USA
关键词： wild rice; seed storability; BSA-seq; genetic resource; rice improvement
期刊名称：GENES （ 影响因子：4.141； 五年影响因子：4.474 ）
ISSN：
年卷期： 2021 年 12 卷 11 期
页码：
收录情况： SCI
摘要： Dongxiang wild rice (Oryza rufipogon Griff.) (DXWR) has strong seed storability and identifying its elite gene resources may facilitate genetic improvements in rice seed storability. In this study, we developed two backcross inbred lines (BILs) populations, with DXWR as a common donor parent and two rice varieties (F6 and R974) as recipient parents. Bulked segregant analysis via whole genome sequencing (BSA-seq) was used to identify seed storability-related loci in the DXWR and F6 population. Two main genomic regions containing 18,550,000-20,870,000 bp on chromosome 4 and 7,860,000-9,780,000 bp on chromosome 9 were identified as candidate loci of DXWR seed storability; these overlapped partially with seed storability-related quantitative trait loci (QTLs) discovered in previous studies, suggesting that these loci may provide important regions for isolating the responsible genes. In total, 448 annotated genes were predicted within the identified regions, of which 274 and 82 had nonsynonymous and frameshift mutations, respectively. We detected extensive metabolic activities and cellular processes during seed storability and confirmed the effects of the seed storability-related candidate loci using four BILs from DXWR and R974. These results may facilitate the cloning of DXWR seed storability-related genes, thereby elucidating rice seed storability and its improvement potential.
- 相关文献
作者其他论文 更多>>
-
Identification of candidate genes controlling cold tolerance at the early seedling stage from Dongxiang wild rice by QTL mapping, BSA-Seq and RNA-Seq
作者：Zhou, Shiqi;Wu, Ting;Li, Xia;Wang, Shilin;Hu, Biaolin
关键词：Dongxiang wild rice; Cold tolerance; RNA-seq; BSA-seq; Gene interaction/coexpression network
-
Genetic Analysis of Novel Fertility Restoration Genes (qRf3 and qRf6) in Dongxiang Wild Rice Using GradedPool-Seq Mapping and QTL-Seq Correlation Analysis
作者：Cai, Wenshan;Li, Wanlin;Duan, Liuying;Chen, Yaling;Zhang, Fantao;Xie, Jiankun;Hu, Biaolin
关键词：fertility restoration; Dongxiang wild rice; QTL; GradedPool-Seq
-
Flavonoid Metabolic Profiles and Gene Mapping of Rice (Oryza sativa L.) Purple Gradient Grain Hulls
作者：Zhang, Fantao;Yang, Limin;Huang, Wenxue;Luo, Xiangdong;Xie, Jiankun;Chen, Yaling;Hu, Biaolin
关键词：Rice; Purple gradient grain hull; Cyanidin; BSA-seq; Flavonoids
-
Development and Characterization of Chromosome Segment Substitution Lines Derived from Oryza rufipogon in the Background of the Oryza sativa indica Restorer Line R974
作者：Ding, Gumu;Zhou, Yi;Zhao, Minmin;Zhang, Fantao;Hu, Biaolin;Yang, Wanling;Xie, Jiankun
关键词：wild rice; chromosome segment substitution line; germplasm resource; salt stress tolerance; restorer line
-
Metabolomic Profiling of Dongxiang Wild Rice Under Salinity Demonstrates the Significant Role of Amino Acids in Rice Salt Stress
作者：Chen, Yaling;Huang, Wenxue;Zhang, Fantao;Luo, Xiangdong;Xie, Jiankun;Hu, Biaolin
关键词：salinity; dongxiang wild rice; metabolome; amino acid; L-asparagine
-
QTL Mapping for Cold Tolerance at Early Seedling Stage in Dongxiang Wild Rice (Oryza rufipogon) under Severe Cold Stress
作者：Hu, Biaolin;Wu, Ting;Li, Xia;Wu, Yanshou;Yin, Jianhua;Hu, Biaolin
关键词：Oryza rufipogon Griff.; Low temperature tolerance; Early growth stage; Quantitative trait loci
-
DFN EH7DFN Association Analysis of Arsenic-Induced Straighthead in Rice (Oryza sativa L.) Based on the Selected Population with a Modified Model
作者：Li, Xiaobai;Zhang, Ning;Wu, Dianxing;Li, Xiaobai;Hu, Biaolin;Pan, Xuhao
关键词：
