您好，欢迎访问江西省农业科学院 机构知识库！

Integrated transcriptomic and metabolomic analysis provides insight into the pollen development of CMS-D1 rice

文献类型： 外文期刊

作者： Wang, Jie 1 ; Ying, Suping 2 ; Long, Weixiong 1 ; Luo, Lihua 1 ; Qian, Mingjuan 1 ; Chen, Wei 1 ; Luo, Laiyang 1 ; Xu, Weibiao 1 ; Li, Yonghui 1 ; Cai, Yaohui 1 ; Peng, Xiaojue 2 ; Xie, Hongwei 1 ;

作者机构： 1.Jiangxi Acad Agr Sci, Jiangxi Superrice Res & Dev Ctr, Natl Engn Res Ctr Rice, Jiangxi Prov Key Lab Rice Germplasm Innovat & Bree, Nanchang 330200, Peoples R China

2.Nanchang Univ, Coll Life Sci, Key Lab Mol Biol & Gene Engn Jiangxi Prov, Nanchang 330031, Peoples R China

关键词： Cytoplasmic male sterility; CMS-D1; Transcriptome; Metabolome; Jasmonic acid

期刊名称：BMC PLANT BIOLOGY （ 影响因子：4.3； 五年影响因子：5.2 ）

ISSN： 1471-2229

年卷期： 2024 年 24 卷 1 期

页码：

收录情况： SCI

摘要： Background Cytoplasmic male sterility (CMS) has greatly improved the utilization of heterosis in crops due to the absence of functional male gametophyte. The newly developed sporophytic D1 type CMS (CMS-D1) rice exhibits unique characteristics compared to the well-known sporophytic CMS-WA line, making it a valuable resource for rice breeding.Results In this research, a novel CMS-D1 line named Xingye A (XYA) was established, characterized by small, transparent, and shriveled anthers. Histological and terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) assays conducted on anthers from XYA and its maintainer line XYB revealed that male sterility in XYA is a result of delayed degradation of tapetal cells and abnormal programmed cell death (PCD) of microspores. Transcriptome analysis of young panicles revealed that differentially expressed genes (DEGs) in XYA, compared to XYB, were significantly enriched in processes related to chromatin structure and nucleosomes during the microspore mother cell (MMC) stage. Conversely, processes associated with sporopollenin biosynthesis, pollen exine formation, chitinase activity, and pollen wall assembly were enriched during the meiosis stage. Metabolome analysis identified 176 specific differentially accumulated metabolites (DAMs) during the meiosis stage, enriched in pathways such as alpha-linoleic acid metabolism, flavone and flavonol biosynthesis, and linolenic acid metabolism. Integration of transcriptomic and metabolomic data underscored the jasmonic acid (JA) biosynthesis pathway was significant enriched in XYA during the meiosis stage compared to XYB. Furthermore, levels of JA, MeJA, OPC4, OPDA, and JA-Ile were all higher in XYA than in XYB at the meiosis stage.Conclusions These findings emphasize the involvement of the JA biosynthetic pathway in pollen development in the CMS-D1 line, providing a foundation for further exploration of the molecular mechanisms involved in CMS-D1 sterility.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：江西省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台