Lateral flow immunoassay based on aggregation induced emission nanobeads for the sensitive and accurate detection of chloramphenicol in pig hair
文献类型： 外文期刊
作者： Lai, Keyang 3 ; Xu, Jun 2 ; Luo, Kai 4 ; Xie, Min 2 ; Chen, Yuan 4 ; Li, Fan 2 ; Zhou, Yaomin 2 ; Gong, Lihui 2 ; Xiong, Yonghua 1 ; Lai, Weihua 1 ;
作者机构： 1.Nanchang Univ, State Key Lab Food Sci & Resources, Nanchang 330047, Peoples R China
2.Jiangxi Acad Agr Sci, Nanchang 330200, Peoples R China
3.Wageningen Univ & Res, NL-6700AA Wageningen, Netherlands
4.Jiangxi Weibang Biotechnol Co, Nanchang 330200, Peoples R China
关键词： Chloramphenicol; Pig hair; Lateral flow immunoassay; Aggregation induced emission fluorescent; nanobeads
期刊名称：ANALYTICAL BIOCHEMISTRY （ 影响因子：2.5； 五年影响因子：2.7 ）
ISSN： 0003-2697
年卷期： 2025 年 698 卷
页码：
收录情况： SCI
摘要： Chloramphenicol (CAP) is a once widely used antibiotic, which is able to cause great harm to human health, is banned in some countries or organizations such as China, USA and the European Union for animal breeding. Because CAP in pig hair degraded slower and had amount of residues, pig hair could be used as the target to detect CAP residues. In this study, a competitive lateral flow immunoassay (LFIA), whose label was aggregation induced emission fluorescent nanobeads (AIEFN), was firstly developed for the detection of CAP in pig hair samples. It exhibited a low limit of detection (0.001 mu g/kg) and a broad linear range (0.0025-0.32 mu g/kg). The recovery rate and coefficient variation with spiked pig hair samples were 88.50%-106.17 % and 1.01%-7.37 %, which indicated good accuracy of this assay. The result of AIEFN-LFIA for the detection of CAP in pig hair was consistent with that of liquid chromatography-mass spectrometry. This assay was rapid with the total detection duration of about 20 min. AIEFN-LFIA was able to be used for rapid, sensitive, accurate and convenient detection of CAP residues in pig hair samples.
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