Effective Agrobacterium-Mediated Transformation System for Eureka Lemon Using Whole Cotyledonary Node
文献类型： 外文期刊
作者： Zhao, Jinfa 1 ; Chen, Yuan 1 ; Wang, Jiajun 1 ; Wang, Chunqing 2 ; Zhou, Yan 1 ;
作者机构： 1.Southwest Univ, Sci City Citrus Res Inst, Integrat Sci Ctr Germplasm Creat Western China Cho, Chongqing 400712, Peoples R China
2.Jiangxi Acad Agr Sci, Inst Plant Protect, Nanchang 330200, Peoples R China
关键词： lemon transformation; whole cotyledonary node; explant; transformation efficiency
期刊名称：PLANTS-BASEL （ 影响因子：4.1； 五年影响因子：4.5 ）
ISSN： 2223-7747
年卷期： 2025 年 14 卷 11 期
页码：
收录情况： SCI
摘要： Agrobacterium-mediated transformation systems using epicotyl explants have been widely used for genetic transformations of citrus. However, their application in lemons is severely constrained by browning of epicotyl tissues, which leads to an extremely low efficiency of transformation. In this study, we developed an optimized Agrobacterium-mediated transformation system using whole cotyledonary node explants of 'Eureka' lemon (Citrus limon), which significantly reduced tissue browning and enhanced transformation efficiency up to 14.48%. In addition, preparation of the whole cotyledonary node was simple and rapid, which reduced time and labor. This system facilitated efficient generation of transgenic lemon plantlets and provided a novel explant source for citrus transformation.
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