Effects of enzyme treatment on the antihypertensive activity and protein structure of black sesame seed (Sesamum indicum L.) after fermentation pretreatment
文献类型： 外文期刊
作者： Du, Tonghao 1 ; Huang, Jinqing 3 ; Xiong, Shijin 1 ; Zhang, Linli 1 ; Xu, Xiaoyan 1 ; Xu, Yazhou 1 ; Peng, Fei 1 ; Huang, Tao 1 ; Xiao, Muyan 1 ; Xiong, Tao 1 ;
作者机构： 1.Nanchang Univ, Sch Food Sci & Technol, 235 Nanjing East Rd, Nanchang 330047, Jiangxi, Peoples R China
2.Nanchang Univ, State Key Lab Food Sci & Resources, 235 Nanjing East Rd, Nanchang 330047, Jiangxi, Peoples R China
3.Jiangxi Acad Agr Sci, Inst Agr Prod Proc, 602 Nanlian Rd, Nanchang 330200, Peoples R China
4.Nanchang Univ, Int Inst Food Innovat, Luozhu Rd,Xiaolan Econ & Technol Dev Zone, Nanchang 330052, Peoples R China
关键词： Black sesame seed; Fermentation pretreatment; Enzyme treatment; ACE inhibition; Antioxidant activity; Protein structure
期刊名称：FOOD CHEMISTRY （ 影响因子：8.8； 五年影响因子：8.6 ）
ISSN： 0308-8146
年卷期： 2023 年 428 卷
页码：
收录情况： SCI
摘要： Effects of enzyme treatment on the hypertensive potential and protein structure of black sesame seed (BSS) were investigated. Compared with BSS, Angiotensin-converting enzyme (ACE) inhibition of fermented black sesame seed (FBSS) has significantly improved after acid protease processing and reached 75.39% at 2 U/g in 3 h. Meanwhile, the zinc chelating ability and antioxidant activity of FBSS hydrolysate as well as surface hydrophobicity, free sulfhydryl content, and peptide content of FBSS protein, were significantly increased. The results illustrated that this strategy promoted the protein unfolding and exposure of hydrophobic residues, thus contributing toward enzymatic hydrolysis. Secondary structure results indicated that the & alpha;-helix of FBSS protein and & beta;-sheet of BSS protein decreased after hydrolyzing. The differences in ACE inhibition may also result from the difference in peptide sequence except for peptide content. In conclusion, the combination of fermentation pretreatment and enzyme treatment is an effective method to enhance the antihypertensive potential of BSS.
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