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SiMYB3 in Foxtail Millet (Setaria italica) Confers Tolerance to Low-Nitrogen Stress by Regulating Root Growth in Transgenic Plants

文献类型： 外文期刊

作者： Ge, Linhao 1 ; Dou, Yining 1 ; Li, Maomao 2 ; Qu, Pengju 1 ; He, Zhang 1 ; Liu, Y. 3 ; Xu, Zhaoshi 1 ; Chen, Jun 1 ; Chen, M 1 ;

作者机构： 1.Chinese Acad Agr Sci, Natl Key Facil Crop Genet Resources & Genet Impro, Key Lab Crop Genet & Breeding, Minist Agr,Inst Crop Sci, Beijing 100081, Peoples R China

2.Jiangxi Acad Agr Sci, Rice Natl Engn Lab, Rice Res Inst, Nanchang 330200, Jiangxi, Peoples R China

3.Xiangyang Acad Agr Sci, Xiangyang 441057, Peoples R China

关键词： foxtail millet; low nitrogen stress; transcriptome analysis; MYB-like transcription factor; transgenic plants

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.923； 五年影响因子：6.132 ）

ISSN：

年卷期： 2019 年 20 卷 22 期

页码：

收录情况： SCI

摘要： Foxtail millet (Setaria italica), which originated in China, has a strong tolerance to low nutrition stresses. However, the mechanism of foxtail millet tolerance to low-nitrogen stress is still unknown. In this study, the transcriptome of foxtail millet under low-nitrogen stress was systematically analyzed. Expression of 1891 genes was altered, including 1318 up-regulated genes and 573 down-regulated genes. KEGG (Kyoto Encyclopedia of Genes and Genomes) analysis revealed that 3% of these genes were involved in membrane transport and 5% were involved in redox processes. There were 74 total transcription factor (TF) genes in the DEGs (differentially expressed genes), and MYB-like transcription factors accounted for one-third (25) of the TF genes. We systematically analyzed the characteristics, expression patterns, chromosome locations, and protein structures of 25 MYB-like genes. The analysis of gene function showed that Arabidopsis and rice overexpressing SiMYB3 had better root development than WT under low-nitrogen stress. Moreover, EMSA results showed that SiMYB3 protein could specifically bind MYB elements in the promoter region of TAR2, an auxin synthesis related gene and MYB3-TAR2 regulate pair conserved in rice and foxtail millet. These results suggested that SiMYB3 can regulate root development by regulating plant root auxin synthesis under low-nitrogen conditions.

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