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Overexpression of MYB-like transcription factor SiMYB30 from foxtail millet (Setaria italica L.) confers tolerance to low nitrogen stress in transgenic rice

文献类型： 外文期刊

作者： Zhang, Yuewei 1 ; He, Zhang 2 ; Qi, Xin 3 ; Li, Maomao 4 ; Liu, Jin 4 ; Le, Si 4 ; Chen, Kai 1 ; Wang, Chunxiao 1 ; Zhou, Yongbin 1 ; Xu, Zhaoshi 1 ; Chen, Jun 1 ; Guo, Changhong 2 ; Tang, Wensi 1 ; Ma, Youzhi 1 ; Chen, Ming 1 ;

作者机构： 1.Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improve, Beijing 100081, Peoples R China

2.Harbin Normal Univ, Coll Life Sci & Technol, Key Lab Mol Cytogenet & Genet Breeding Heilongjian, Harbin 150025, Heilongjiang, Peoples R China

3.Tianjin Acad Agr Sci, Inst Germplasm Resources & Biotechnol, Tianjin 300112, Peoples R China

4.Jiangxi Acad Agr Sci, Res Ctr Jiangxi Crop Germplasm Resources, Nanchang 330200, Peoples R China

关键词： Foxtail millet; Low nitrogen; MYB-Like transcription factor; Nitrogen use efficiency; Transgenic rice

期刊名称：PLANT PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：6.5； 五年影响因子：6.4 ）

ISSN： 0981-9428

年卷期： 2023 年 196 卷

页码：

收录情况： SCI

摘要： Nitrogen fertilizers significantly increase crop yield; however, the negative impact of excessive nitrogen use on the environment and soil requires urgent attention. Improving crop nitrogen use efficiency (NUE) is crucial to increase yields and protect the environment. Foxtail millet (Setaria italica L.), a gramineous crop with significant tolerance to barren croplands, is an ideal model crop for studying abiotic stress resistance in gramineous crops. However, knowledge of the regulatory network for NUE in foxtail millet is fragmentary. Herein, we identified an R2R3-like MYB transcription factor in foxtail millet, SiMYB30, which belongs to MYB subfamily 17. The expression of SiMYB30 is responsive to low nitrogen (LN) concentration. Compared with wildtype Kitaake, seedlings of rice lines overexpressing SiMYB30 showed significantly increased shoot fresh and dry weights, plant height, and root area under LN treatment indoors. Consistently, overexpression of SiMYB30 in field experiments significantly increased grain and stem nitrogen contents, grain yield per plant, and stem weight in rice. Furthermore, qRT-PCR revealed that SiMYB30 effectively activated the expression of nitrogen uptake-related genes-OsNRT1, OsNRT1.1B, and OsNPF2.4-and nitrogen assimilation-related genes-OsGOGAT1, OsGO-GAT2, and OsNIA2. Notably, SiMYB30 directly bound to the promoter of OsGOGAT2 and regulated its expres-sion. These results highlight the novel and pivotal role of SiMYB30 in improving crop NUE.

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