文献类型: 外文期刊
作者: Qi, Liying 1 ; Chen, Ling 1 ; Wang, Chuansen 1 ; Zhang, Shaoling 3 ; Yang, Yingjie 1 ; Liu, Jianlong 1 ; Li, Dingli 1 ; S 1 ;
作者机构: 1.Qingdao Agr Univ, Coll Hort, Qingdao Key Lab Genet Improvement & Breeding Hort, Qingdao 266109, Peoples R China
2.Jiangxi Acad Agr Sci, Inst Soil & Fertilizer & Resource & Environm, Nanchang 330200, Peoples R China
3.Nanjing Agr Univ, Coll Hort, Nanjing 210095, Peoples R China
关键词: Pyrus; auxin transport; dwarfing
期刊名称:PLANTS-BASEL ( 影响因子:3.935; )
ISSN:
年卷期: 2020 年 9 卷 3 期
页码:
收录情况: SCI
摘要: PIN-FORMED (PIN) encodes a key auxin polar transport family that plays a crucial role in the outward transport of auxin and several growth and development processes, including dwarfing trees. We identified a dwarfing pear rootstock 'OHF51' (Pyrus communis), which limits the growth vigor of the 'Xueqing' (Pyrus bretschneideri x Pyrus pyrifolia) scion, and isolated 14 putative PbPINs from the pear Pyrus bretschneideri. The phylogenic relationships, structure, promoter regions, and expression patterns were analyzed. PbPINs were classified into two main groups based on the protein domain structure and categorized into three major groups using the neighbor-joining algorithm. Promoter analysis demonstrated that PbPINs might be closely related to plant growth and development. Through quantitative real-time PCR (qRT-PCR) analysis, we found that the expression patterns of 14 PbPINs varied upon exposure to different organs in dwarfing and vigorous stocks, 'OHF51' and 'QN101' (Pyrus betulifolia), indicating that they might play varying roles in different tissues and participated in the regulation of growth vigor. These results provide fundamental insights into the characteristics and evolution of the PINs family, as well as the possible relationship between dwarfing ability and auxin polar transport.
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关键词:chlorotic leaf spot; detection; Emaravirus; etiology; fruit; genome; Pyrus pyrifolia; tree fruit; viruses and viroids