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Identification and Characterization of a Pear Chlorotic Leaf Spot-Associated Virus, a Novel Emaravirus Associated with a Severe Disease of Pear Trees in China

文献类型： 外文期刊

作者： Liu, Huazhen 1 ; Wang, Guoping 1 ; Yang, Zuokun 1 ; Wang, Yanxiang 1 ; Zhang, Zhe 1 ; Li, Longhui 1 ; Waqas, Muhammad; 1 ;

作者机构： 1.Huazhong Agr Univ, Coll Plant Sci & Technol, Key Lab Crop Dis Monitoring & Safety Control Hube, Wuhan 430070, Hubei, Peoples R China

2.Huazhong Agr Univ, Key Lab Hort Plant Biol, Minist Educ, Wuhan 430070, Hubei, Peoples R China

3.Zhejiang Univ, Anal Ctr Agrobiol & Environm Sci, Hangzhou 317502, Zhejiang, Peoples R China

4.Hubei Acad Agr Sci, Res Inst Fruit & Tea, Natl Sand Pear Germplasm Repository Wuchang, Wuhan 430064, Hubei, Peoples R China

5.Jiangxi Acad Agr Sci, Nanchang 330200, Jiangxi, Peoples R China

关键词： chlorotic leaf spot; detection; Emaravirus; etiology; fruit; genome; Pyrus pyrifolia; tree fruit; viruses and viroids

期刊名称：PLANT DISEASE （ 影响因子：4.438； 五年影响因子：4.7 ）

ISSN： 0191-2917

年卷期： 2020 年 104 卷 11 期

页码：

收录情况： SCI

摘要： Pear chlorotic leaf spot (PCLS) is a recently emerged disease of commercially cultivated sandy pear (Pyrus pyrifolia) trees in central and southern China. By integrating high-throughput sequencing and conventional Sanger sequencing of reverse-transcription (RT)-PCR products, a novel emaravirus infecting pear trees was identified and molecularly characterized. The virus was provisionally named pear chlorotic leaf spot-associated virus (PCLSaV). PCLSaV shows the typical molecular features of members of the genus Emaravirus in the family Fimoviridae. It has a genome composed of at least five negative-sense RNA segments, with each containing a single open reading frame and two complementary 13-nucleotide stretches at the 5' and 3' termini. PCLSaV shows a close phylogenetic relationship with recognized emaraviruses but forms a separate Glade. Moreover, double-membrane-bound bodies were observed in PCLSaV-infected tissues and in extracts of PCLSaV-infected leaves. For the first time, our study revealed the profile distribution of viral RNA reads from the RNA-seq libraries of three samples along the RNA1 to RNA5 of an emaravirus. Field surveys combined with specific RT-PCR assays revealed the presence of PCLSaV in almost all PCLS-diseased pear samples, strongly supporting the association of the virus with the PCLS disease. This study revealed the first emaravirus infecting pear trees and its association with a severe pear chlorotic leaf disease.

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