您好，欢迎访问江西省农业科学院 机构知识库！

Transgenic double-stranded RNA rice, a potential strategy for controlling striped stem borer (Chilo suppressalis)

文献类型： 外文期刊

作者： Mao, Cui 2 ; Zhu, Xiaoping 2 ; Wang, Peipei 1 ; Sun, Yajie 1 ; Huang, Renliang 3 ; Zhao, Mingchao 2 ; Hull, J. Joe 4 ; Lin, Yongjun 2 ; Zhou, Fei 2 ; Chen, Hao 2 ; Ma, Weihua 1 ;

作者机构： 1.Huazhong Agr Univ, Coll Plant Sci & Technol, Wuhan, Peoples R China

2.Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan, Peoples R China

3.Jiangxi Acad Agr Sci, Rice Res Inst, Key Lab Rice Physiol & Genet Jiangxi Prov, Nanchang Subctr Rice Natl Engn Lab, Nanchang, Jiangxi, Peoples R China

4.USDA ARS, Pest Management & Biocontrol Res Unit, US Arid Land Agr Res Ctr, Maricopa, AZ USA

关键词： Chilo suppressalis; CssHsp; double-stranded RNA; insect-resistant rice; pest control

期刊名称：PEST MANAGEMENT SCIENCE （ 影响因子：4.462； 五年影响因子：4.688 ）

ISSN： 1526-498X

年卷期： 2022 年 78 卷 2 期

页码：

收录情况： SCI

摘要： BACKGROUND Although the striped stem borer (SSB, Chilo suppressalis Walker) is a devastating pest of rice that causes significant economic losses, management options are currently limited. Plant-mediated RNA interference (RNAi) is an emerging crop protection technique in which transgenic plants are modified to express insect-specific double-stranded RNAs (dsRNAs) that trigger RNAi silencing in target pests. RESULT In this study, an RNAi-based screen of 35 candidate SSB genes identified a small heat shock protein gene (CssHsp) as a potential plant-based RNAi target. To assess its utility in planta, a total of 39 transgenic rice plants were generated, with 11 independent transformants found to contain a single copy of the dsCssHsp expression cassette. In life-time feeding bioassays, three transgenic lines (DS10, DS35, DS36) were found to have significant negative impacts on SSB populations. After feeding for 8 days, mortality in the three transgenic lines exceeded 60%. By pupation, mortality further increased to 90% and few SSB survived to eclosion. Gene expression analyses confirmed that CssHsp transcript levels were significantly reduced after feeding on the transgenic dsCssHsp rice. CONCLUSION These results demonstrate the potential for developing a plant-mediated RNAi strategy targeting CssHsp as a more biorational field-based approach for SSB control.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：江西省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台