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The chimeric mitochondrial gene orf182 causes non-pollen-type abortion in Dongxiang cytoplasmic male-sterile rice

文献类型： 外文期刊

作者： Xie, Hongwei 1 ; Peng, Xiaojue 2 ; Qian, Mingjuan 1 ; Cai, Yicong 4 ; Ding, Xia 2 ; Chen, Qiusheng 2 ; Cai, Qiying 2 ; Zh 1 ;

作者机构： 1.Jiangxi Acad Agr Sci, Natl Engn Lab Rice, Jiangxi Super Rice Res & Dev Ctr, Nanchang, Jiangxi, Peoples R China

2.Nanchang Univ, Sch Life Sci, Nanchang, Jiangxi, Peoples R China

3.Nanchang Univ, Inst Life Sci, Nanchang, Jiangxi, Peoples R China

4.China Natl Rice Res Inst, Hangzhou, Zhejiang, Peoples R China

关键词： cytoplasmic male sterility; mitochondrial genome; orf182; mitotype-specific sequences; Dongxiang wild rice; D1-CMS

期刊名称：PLANT JOURNAL （ 影响因子：6.417； 五年影响因子：7.627 ）

ISSN： 0960-7412

年卷期： 2018 年 95 卷 4 期

页码：

收录情况： SCI

摘要： D1-cytoplasmic male sterility (CMS) rice is a sporophytic cytoplasmic male-sterile rice developed from Dongxiang wild rice that exhibits a no-pollen-grain phenotype. A mitochondrial chimeric gene (orf182) was detected by mitochondrial genome sequencing and a comparative analysis. Orf182 is composed of three recombinant fragments, the largest of which is homologous to Sorghum bicolor mitochondrial sequences. In addition, orf182 was found only in wild rice species collected from China. Northern blot analysis showed that orf182 transcripts were affected by Rf genes in the isocytoplasmic restorer line DR7. Western blot analysis showed that the ORF182 product was localized in the mitochondria of the CMS line. An expression cassette containing orf182 fused to a mitochondrial transit peptide induced the maintainer line of male sterility, which lacked pollen grains in the anthers. Furthermore, the invivo expression of orf182 also inhibited the growth of Escherichia coli, with lower respiration rate, excess accumulation of reactive oxygen species and decreased ATP levels. We conclude that the mitochondrial chimeric gene orf182 possesses a unique structure and origin differing from other identified mitochondrial CMS genes, and this gene is connected to non-pollen type of sporophytic male sterility in D1-CMS rice.

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