文献类型： 外文期刊
作者： Fan, Junxia 1 ; Zhang, Huanxin 3 ; Li, Yuzhen 1 ; Chen, Ziming 1 ; Chen, Tianming 1 ; Zeng, Bin 1 ; Zhang, Zhe 1 ;
作者机构： 1.Jiangxi Sci & Technol Normal Univ, Coll Life Sci, Jiangxi Key Lab Bioproc Engn, Nanchang 330013, Jiangxi, Peoples R China
2.Jiangxi Sci & Technol Normal Univ, Coll Life Sci, Coinnovat Ctr In Vitro Diagnost Reagents & Device, Nanchang 330013, Jiangxi, Peoples R China
3.Jiangxi Acad Agr Sci, Inst Hort, Nanchang 330200, Jiangxi, Peoples R China
4.Shenzhen Technol Univ, Coll Pharm, Shenzhen 518118, Peoples R China
关键词： Nramp; Aspergillus oryzae; Transporter
期刊名称：3 BIOTECH （ 影响因子：2.406； 五年影响因子：3.203 ）
ISSN： 2190-572X
年卷期： 2021 年 11 卷 10 期
页码：
收录情况： SCI
摘要： The Nramp (natural resistance-associated macrophage protein) family of genes has been identified and characterized widely in many species. However, the Nramp genes and their characterizations have not been reported for Aspergillus oryzae. Here, only one Nramp gene AoNramp1 in A. oryzae genome was identified. Phylogenetic analysis revealed that AoNramp1 is not clustered with Nramps from yeast genus. Expression analysis showed that the transcript level of AoNramp1 was strongly induced under both Zn/Mn-replete and -deplete conditions. The GUS-staining assay indicated that the expression of AoNramp1 was strongly induced by Zn/Mn. Moreover, the AoNramp1 deletion and overexpression strains were constructed by the CRISPR/Cas9 system and A. oryzae amyB promoter, respectively. Phenotypic analysis showed that overexpression and deletion of AoNramp1 caused growth defects under Zn/Mn-deplete and -replete conditions, including mycelium growth and conidia formation. Together, these findings provide valuable information for further study on the biological roles of AoNramp1 in A. oryzae.
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