Influence of a rumen ciliate-derived xylanase on the gut microbiota composition: A potential enzyme for prebiotic applications
文献类型： 外文期刊
作者： Su, Weide 1 ; Luo, Huiying 3 ; Chen, Xiaolian 1 ; Ai, Gaoxiang 1 ; Wei, Qipeng 1 ; Zou, Zhiheng 1 ; Xu, Chuanhui 1 ; Chen, Jiang 1 ; Xiong, Pingwen 1 ; Song, Wenjing 1 ; Song, Qiongli 1 ;
作者机构： 1.Jiangxi Acad Agr Sci, Inst Anim Husb & Vet Med, Nanchang 330200, Peoples R China
2.Jiangxi Prov Key Lab Anim Green & Hlth Breeding, Nanchang 330200, Peoples R China
3.Chinese Acad Agr Sci, Inst Anim Sci, Beijing 100193, Peoples R China
关键词： Xylanase; Xylo-oligosaccharides; Prebiotics; Gut microbiota
期刊名称：ENZYME AND MICROBIAL TECHNOLOGY （ 影响因子：3.7； 五年影响因子：3.4 ）
ISSN： 0141-0229
年卷期： 2025 年 190 卷
页码：
收录情况： SCI
摘要： A xylanase derived from rumen ciliates designated as XynC, was successfully expressed and purified. The enzyme's catalytic properties, including its optimal pH (5.5) and temperature (40 degrees C), were determined, demonstrating high stability under mildly acidic and moderate thermal conditions. The substrate specificity of XynC was investigated by analyzing its hydrolysis pattern with beechwood xylan, where it preferentially degraded higher xylo-oligosaccharides (xylotetrose (X4), xylopentose (X5), xylohexose (X6)) while showing no activity on smaller oligosaccharides like xylobiose (X2) and xylotriose (X3). The in vivo effects of XynC on gut microbiota were evaluated by administering the enzyme to mice, followed by analysis of fecal 16S rRNA to assess shifts in gut bacterial populations. The results revealed dose-dependent modulation of gut microbiota composition, with a significant increase in Lactobacillus abundance observed in the medium- and high-dose groups, while no notable change was detected in the low-dose group. These dose-related microbial responses suggest that the efficacy of XynC in promoting probiotic growth is dependent on administration level. These findings suggest that XynC selectively promotes the growth of beneficial probiotics in a dosage-sensitive manner, supporting its potential as a functional feed additive for enhancing gut health and nutrient utilization in livestock.
- 相关文献
作者其他论文 更多>>
-
Sublancin alleviates Salmonella typhimurium-induced colitis and balances gut bacterial dysbiosis in mice
作者：Yang, Yanzhi;Guo, Bingyu;Chen, Siyuan;Song, Tongxing;Xu, Chuanhui;Song, Tongxing
关键词：Colitis; Sublancin; Gut microbiota; Inflammation;
Salmonella typhimurium -
Structural Characterization and Immune Activation Capacity of Peptidoglycan from Corynebacterium glutamicum in RAW264.7 Cells
作者：Wang, Xiaoying;Li, Shuzhen;Zheng, Aijuan;Chen, Zhimin;Liu, Guohua;Chen, Jiang;Zou, Zhiheng
关键词：
Corynebacterium glutamicum ; peptidoglycan; immune activation; RAW264.7 cells; cytokines -
Lactobacillus plantarum-Derived Inorganic Polyphosphate Regulates Immune Function via Inhibiting M1 Polarization and Resisting Oxidative Stress in Macrophages
作者：Li, Shuzhen;Zheng, Aijuan;Chen, Zhimin;Wang, Xiaoying;Liu, Guohua;Chen, Jiang;Zou, Zhiheng
关键词：inorganic polyphosphate; macrophages; oxidative stress; M1 polarization; immunomodulation
-
Unveiling the sweet culprit: Excessive fructose intake leading to kidney injury through hypoxanthine accumulation
作者：Ai, Gaoxiang;Chen, Xiaolian;Ai, Gaoxiang;Chen, Xiaolian;Ai, Gaoxiang;Tan, Wenwen;Huang, Ziwei;Wu, Xiaoyan;Su, Ziren;Chen, Jiannan;Chen, Xiaolian;Xu, Lieqing;Yu, Qiuxia;Xie, Jianhui;Xie, Jianhui;Dou, Yaoxing;Dou, Yaoxing
关键词：Fructose; Hypoxanthine; Kidney injury; Keap1-Nrf2 signaling pathway; NLRP3 inflammasome
-
LC/MS-based lipidomics and transcriptomics reveal lipid diversity and regulatory networks underlying intramuscular fat differences in Xingguo grey geese
作者：Zou, Mengyun;Zhang, Weihong;Xiong, Xiaolan;Rao, Linjie;Wei, Qipeng;Li, Haiqin;Zhang, Fanfan;Xie, Jinfang;Huang, Jiangnan;Wang, Zhangzhang
关键词：LC/MS-based lipidomics; Transcriptomics; Intramuscular fat; Xingguo grey goose; Breast muscle
-
Immune-iron homeostasis deciphers resistance divergence to Riemerella anatipestifer in ducks
作者：Zou, Mengyun;Xiong, Xiaolan;Zhang, Weihong;Wei, Yue;Tang, Weiguo;Wei, Qipeng;Huang, Cong;Kang, Zhaofeng;Xie, Jinfang;Huang, Jiangnan
关键词：duck;
Riemerella anatipestifer ; disease resistance; immune response; iron metabolism -
Mechanism Analysis of UCP2 During the Oxidative Stress Injury of Intestinal Porcine Epithelial Cell Line-J2
作者：Su, Weide;Xu, Chuanhui;Jiang, Hongping;Song, Wenjing;Xiong, Pingwen;Chen, Jiang;Ai, Gaoxiang;Song, Qiongli;Zou, Zhiheng;Wei, Qipeng;Chen, Xiaolian;Su, Weide;Xu, Chuanhui;Jiang, Hongping;Song, Wenjing;Xiong, Pingwen;Chen, Jiang;Ai, Gaoxiang;Song, Qiongli;Zou, Zhiheng;Wei, Qipeng;Chen, Xiaolian
关键词：UCP2; oxidative stress; porcine intestinal epithelial cells; genipin; apoptosis
