Integrated Metagenomics and 15N Isotope Tracing Reveal the Mechanisms Through which the Nitrogen-Planting Density Interaction Impacts Rice Root Nitrogen Uptake Efficiency
文献类型： 外文期刊
作者： Sun, Changhui 1 ; Lu, Yanyao 1 ; Tang, Guoping 3 ; Wang, Runnan 1 ; Wu, Han 1 ; Zhang, Jiao 1 ; Cai, Shuo 4 ; Zhu, Jinyan 1 ; Xiong, Qiangqiang 1 ;
作者机构： 1.Yangzhou Univ, Jiangsu Key Lab Crop Genet & Physiol, Jiangsu Key Lab Crop Cultivat & Physiol, Agr Coll, Yangzhou 225009, Peoples R China
2.Yangzhou Univ, Jiangsu Coinnovat Ctr Modern Prod Technol Grain Cr, Yangzhou 225009, Peoples R China
3.Jiangxi Acad Agr Sci, Rice Res Inst, Nanchang 330045, Peoples R China
4.Jiangxi Irrigat Expt Cent Stn, Nanchang 330045, Peoples R China
关键词： Rice; Density; Nitrogen Uptake Efficiency; Enzyme Activity; Isotope Tracing; Metagenomics
期刊名称：JOURNAL OF SOIL SCIENCE AND PLANT NUTRITION （ 影响因子：3.9； 五年影响因子：3.9 ）
ISSN： 0718-9508
年卷期： 2024 年
页码：
收录情况： SCI
摘要： Designing appropriate nitrogen (N) application rates and planting densities is crucial for ensuring high-yield and high-quality rice production. The effects of N fertilizer and planting density on the soil microbial community composition and abundance and on root N uptake mechanisms were investigated. In this study, high-nitrogen low-density (HNLD), medium-nitrogen medium-density (MNMD) and low-nitrogen high-density (LNHD) experiments were conducted via the bucket planting method, and plant physiology, dry matter mass and N uptake, root enzyme activities and rhizosphere soil microbial communities were examined via metagenomic sequencing and isotope tracing. The HNLD treatment exhibited the highest N absorption and utilization capacity. Compared to those in the other treatments, the delta N-15 values and abundance increased by 52.96-63.45% during the maturation stage and by 34.48-48.36% during the heading stage. Compared with those of the other treatments, the Ndff (N-15 derived from fertilizer) increased by 59.33-60.92% during the maturation stage and by 40.24-47.56% during the heading stage. Actinobacteria, Acidobacteria, Chloroflexi, and Proteobacteria were confirmed to be the dominant bacterial groups among the treatments. Based on these findings, a correlation-oriented model and dual-factor correlation network were constructed, focusing on the variations in root nitrite reductase (NIR) activity, rhizosphere soil sucrase (S-SC) and soil catalase (S-CAT) activities, and Actinobacteria and Proteobacteria communities. Overall, this study provides theoretical support for determining N application rates and planting densities in practical rice production, with the ultimate goal of enhancing root N uptake efficiency.
- 相关文献
作者其他论文 更多>>
-
Wild species rice OsCERK1DY-mediated arbuscular mycorrhiza symbiosis boosts yield and nutrient use efficiency in rice breeding
作者：Han, Ruicai;Yang, Zhou;Zhu, Shan;Tang, Guoping;Shen, Xianhua;Huang, Renliang;Duanmu, Deqiang;Cao, Yangrong;Wang, Chunquan
关键词：OsCERK1; Natural variation; Arbuscular mycorrhizal; Rice yield
-
MG1 interacts with a protease inhibitor and confers resistance to rice root-knot nematode
作者：Wang, Xiaomin;Cheng, Rui;Xu, Daochao;Jin, Liang;Guo, Xiaoli;Huang, Renliang;Li, Haoxing;Wu, Yufeng;Tang, Jiuyou;Chu, Chengcai;Sun, Changhui;Peng, Deliang
关键词：
-
Integrated 16S and metabolomics revealed the mechanism of drought resistance and nitrogen uptake in rice at the heading stage under different nitrogen levels
作者：Sun, Changhui;Wang, Runnan;Zhu, Jinyan;Xiong, Qiangqiang;Sun, Changhui;Wang, Runnan;Zhu, Jinyan;Xiong, Qiangqiang;Tang, Guoping;Cai, Shuo;Shi, Hong;Liu, Fangping;Xie, Hengwang;Xiong, Qiangqiang;Zhu, Jinyan;Xiong, Qiangqiang
关键词：rice; heading date; nitrogen; drought; 16S rRNA; metabolomics
-
Transcriptome and Metabolome Analysis Provides Insights into the Heterosis of Yield and Quality Traits in Two Hybrid Rice Varieties (Oryza sativa L.)
作者：Zhou, Dahu;Zhou, Xinyi;Liu, Lin;He, Haohua;Sun, Changhui;Chen, Le;Xiong, Qiangqiang;Tang, Guoping
关键词：heterosis; transcriptome; metabolome; hybrid rice; WGCNA
-
Effects of nitrogen application after abrupt drought-flood alternation on rice root nitrogen uptake and rhizosphere soil microbial diversity
作者：Zhu, Jinyan;Li, Ao;Zhang, Jiao;Sun, Changhui;Chen, Le;Hu, Jinlong;Zhou, Nianbing;Wang, Shuai;Zhou, Yong;Zhang, Hongcheng;Xiong, Qiangqiang;Zhu, Jinyan;Chen, Le;Hu, Jinlong;Zhou, Nianbing;Zhou, Yong;Zhang, Hongcheng;Xiong, Qiangqiang;Xiong, Qiangqiang;Tang, Guoping
关键词：Transcriptome; 16S rRNA gene; Root; Nitrogen; Abrupt drought-flood alternation
-
Tectorigenin alleviates intrahepatic cholestasis by inhibiting hepatic inflammation and bile accumulation via activation of PPAR gamma
作者：Xiang, Jiaqing;Yang, Guangyan;Wu, Han;Jiang, Lingyun;Liang, Zhen;Kang, Lin;Yang, Shu;Ma, Chuanrui;Ma, Chuanrui;Wei, Lingling;Wu, Han;Zhang, Wei;Tao, Xiuhua;Yang, Shu
关键词：hepatocytes; intrahepatic cholestasis; macrophage; PPARγ tectorigenin
-
Danthron ameliorates obesity and MAFLD through activating the interplay between PPAR?/RXR? heterodimer and adiponectin receptor 2
作者：Ma, Chuanrui;Zhang, Chao;Zhang, Jing;Zhao, Linna;Wu, Han;Kang, Lin;Yang, Shu;Yang, Shu;Ma, Chuanrui;Zhang, Chao;Zhang, Jing;Zhao, Linna;Ma, Chuanrui;Xia, Ronglin;Zhang, Jing;Zhao, Linna;Wu, Han;Xia, Ronglin;Wang, Zhongyan;Wei, Lingling
关键词：Danthron; Obesity; Hepatic steatosis; AdipoR2; AMPK?; PPAR?
