文献类型： 外文期刊

作者： Zhou, Guopeng ¹ ; Fan, Kunkun ² ; Li, Guilong ³ ; Gao, Songjuan ⁴ ; Chang, Danna ¹ ; Liang, Ting ¹ ; Li, Shun ⁴ ; Liang, Hai ⁴ ; Zhang, Jiudong ⁵ ; Che, Zongxian ⁵ ; Cao, Weidong ¹ ;

作者机构： 1.Chinese Acad Agr Sci, Key Lab Plant Nutr & Fertilizer, Minist Agr & Rural Affairs, Inst Agr Resources & Reg Planning, Beijing, Peoples R China

2.Chinese Acad Sci, Inst Soil Sci, State Key Lab Soil & Sustainable Agr, Nanjing, Peoples R China

3.Jiangxi Acad Agr Sci, Inst Soil & Fertilizer & Resource Environm, Nanchang, Peoples R China

4.Nanjing Agr Univ, Coll Resources & Environm Sci, Nanjing, Peoples R China

5.Gansu Acad Agr Sci, Inst Soil Fertilizer & Water Saving Agr, Lanzhou, Peoples R China

6.Chinese Acad Agr Sci, Inst Agr Resources & Reg Planning, Key Lab Plant Nutr & Fertilizer, Minist Agr & Rural Affairs, Beijing 100081, Peoples R China

关键词： farmland system; interspecies relationship; long-term fertilization; key ecological cluster

期刊名称：IMETA （ 影响因子：33.2； 五年影响因子：33.2 ）

ISSN： 2770-5986

年卷期： 2023 年 2 卷 1 期

页码：

收录情况： SCI

摘要： Biological nitrogen (N) fixation (BNF) via diazotrophs is an important ecological process for the conversion of atmospheric N to biologically available N. Although soil diazotrophs play a dominant role in BNF and arbuscular mycorrhizal fungi (AMF) serve as helpers to favor BNF, the response of soil BNF and diazotrophic communities to different long-term fertilizations and the role of AMF in diazotrophs-driven BNF are poorly understood. Herein, a 33-year fertilization experiment in a wheat-maize intercropping system was conducted to investigate the changes in soil BNF rates, diazotrophic and AMF communities, and their interactions after long-term representative fertilization (chemical fertilizer, cow manure, wheat straw, and green manure). We found a remarkable increase in soil BNF rates after more than three decades of fertilization compared with nonfertilized soil, and the green manure treatment rendered the highest enhancement. The functionality strengthening was mainly associated with the increase in the absolute abundance of diazotrophs and AMF and the relative abundance of the key ecological cluster of Module #0 (gained from the co-occurrence network of diazotrophic and AMF species) with dominant diazotrophs such as Skermanella and Azospirillum. Furthermore, although the positive correlations between diazotrophs and AMF were reduced under long-term organic fertilization regimes, green manuring could reverse the decline within Module #0, and this had a positive relationship with the BNF rate. This study suggests that long-term fertilization could promote N fixation and select specific groups of N fixers and their helpers in certain areas. Our work provides solid evidence that N fixation and certain groups of diazotrophic and AMF taxa and their interspecies relationship will be largely favored after the fertilized strategy of green manure. In this study, a 33-year fertilization experiment was conducted to investigate the changes in soil BNF rates, diazotrophic and AMF communities, and their interactions after various long-term fertilizations. We found a remarkable increase in soil BNF rates after more than three decades of fertilization compared with nonfertilized soil, and the green manure treatment rendered the highest enhancement.image Long-term fertilization enhanced soil biological nitrogen fixation (BNF).Diazotrophic and arbuscular mycorrhizal fungal (AMF) abundance is positively linked to BNF.Key ecological cluster consisting of diazotrophs and AMF played vital roles in soil BNF.Long-term fertilization reduced the synergistic functions of diazotrophs and AMF.Green manure strengthened the synergistic function within the key ecological cluster.