文献类型： 外文期刊

作者： Zhou, Siyu ¹ ; Chen, Daipeng ¹ ; Fu, Yujia ¹ ; Zhou, Jingyi ¹ ; Yang, Yingqing ² ; Xie, Changping ¹ ; Zheng, Li ¹ ;

作者机构： 1.Hainan Univ, Sanya Nanfan Res Inst, Hainan Yazhou Bay Seed Lab, Sanya 572025, Peoples R China

2.Hainan Univ, Minist Educ, Key Lab Green Prevent & Control Trop Plant Dis & P, Haikou 570228, Hainan, Peoples R China

3.Hainan Univ, Sch Plant Protect, Haikou 570228, Hainan, Peoples R China

4.Jiangxi Acad Agr Sci, Inst Plant Protect, Nanchang 330200, Peoples R China

期刊名称：ARCHIVES OF VIROLOGY （ 影响因子：2.7； 五年影响因子：2.4 ）

ISSN： 0304-8608

年卷期： 2023 年 168 卷 4 期

页码：

收录情况： SCI

摘要： Here, we report a novel mycotombus-like mycovirus, tentatively named "Phoma matteucciicola RNA virus 2" (PmRV2), derived from the phytopathogenic fungus Phoma matteucciicola strain HNQH1. The complete PmRV2 genome is comprised of a positive-sense single-stranded RNA (+ssRNA) of 3,460 nucleotides (nt) with a GC content of 56.71%. Sequence analysis of PmRV2 indicated the presence of two noncontiguous open reading frames (ORFs) encoding a hypothetical protein and an RNA-dependent RNA polymerase (RdRp), respectively. PmRV2 contains a metal-binding 'GDN' triplet in motif C of RdRp, while most +ssRNA mycoviruses contained a 'GDD' motif in the same region. A BLASTp search showed that the RdRp amino acid sequence of PmRV2 was most closely related to the RdRp of Macrophomina phaseolina umbra-like virus 1 (50.72% identity) and Erysiphe necator umbra-like virus 2 (EnUlV2, 44.84% identity). Phylogenetic analysis indicated that PmRV2 grouped together with EnUlV2 within the recently proposed family "Mycotombusviridae".