Characterization of a novel mycotombus-like virus from the plant-pathogenic fungus Phoma matteucciicola
文献类型： 外文期刊
作者： Zhou, Siyu 1 ; Chen, Daipeng 1 ; Fu, Yujia 1 ; Zhou, Jingyi 1 ; Yang, Yingqing 2 ; Xie, Changping 1 ; Zheng, Li 1 ;
作者机构： 1.Hainan Univ, Sanya Nanfan Res Inst, Hainan Yazhou Bay Seed Lab, Sanya 572025, Peoples R China
2.Hainan Univ, Minist Educ, Key Lab Green Prevent & Control Trop Plant Dis & P, Haikou 570228, Hainan, Peoples R China
3.Hainan Univ, Sch Plant Protect, Haikou 570228, Hainan, Peoples R China
4.Jiangxi Acad Agr Sci, Inst Plant Protect, Nanchang 330200, Peoples R China
期刊名称：ARCHIVES OF VIROLOGY （ 影响因子：2.7； 五年影响因子：2.4 ）
ISSN： 0304-8608
年卷期： 2023 年 168 卷 4 期
页码：
收录情况： SCI
摘要： Here, we report a novel mycotombus-like mycovirus, tentatively named "Phoma matteucciicola RNA virus 2" (PmRV2), derived from the phytopathogenic fungus Phoma matteucciicola strain HNQH1. The complete PmRV2 genome is comprised of a positive-sense single-stranded RNA (+ssRNA) of 3,460 nucleotides (nt) with a GC content of 56.71%. Sequence analysis of PmRV2 indicated the presence of two noncontiguous open reading frames (ORFs) encoding a hypothetical protein and an RNA-dependent RNA polymerase (RdRp), respectively. PmRV2 contains a metal-binding 'GDN' triplet in motif C of RdRp, while most +ssRNA mycoviruses contained a 'GDD' motif in the same region. A BLASTp search showed that the RdRp amino acid sequence of PmRV2 was most closely related to the RdRp of Macrophomina phaseolina umbra-like virus 1 (50.72% identity) and Erysiphe necator umbra-like virus 2 (EnUlV2, 44.84% identity). Phylogenetic analysis indicated that PmRV2 grouped together with EnUlV2 within the recently proposed family "Mycotombusviridae".
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