Expression of beta-defensins in intestines of chickens injected with vitamin D-3 and lipopolysaccharide
文献类型： 外文期刊
作者： Lu, L. 1 ; Li, S. M. 2 ; Zhang, L. 1 ; Liu, X. Q. 1 ; Li, D. Y. 1 ; Zhao, X. L. 1 ; Liu, Y. P. 1 ;
作者机构： 1.Sichuan Agr Univ, Farm Anim Genet Resources Explorat & Innovat Key, Yaan, Peoples R China
2.Jiangxi Acad Agr Sci, Inst Anim Husb & Vet, Nanchang, Peoples R China
关键词： beta-defensin;Chicken;Host defense peptide;Vitamin D3;Lipopolysaccharide
期刊名称：GENETICS AND MOLECULAR RESEARCH （ 影响因子：0.764； 五年影响因子：0.912 ）
ISSN： 1676-5680
年卷期： 2015 年 14 卷 2 期
页码：
收录情况： SCI
摘要： The objective of this study was to evaluate the effect of vitamin D-3 (VD3) on the regulation of chicken intestinal beta-defensin genes under normal and lipopolysaccharides (LPS) conditions. Four treatment groups were used, including a negative control group, VD3-injection group, LPS-injection group, and both VD3-injection and LPS-injection group. At 4, 24, and 48 h post-injection, intestines were collected and RNA was isolated to measure the chicken beta-defensin genes with putative vitamin D responsive elements using quantitative polymerase chain reaction. Expressions of all 7 chicken beta-defensin genes was detectable in the intestines. Significant increases in GAL-6, -7 and -9 were found following LPS injection treatment at 4, 24, and 48 h post-injection, respectively, whereas VD3 injection did not affect the expression of any investigated genes under normal conditions. However, the expression of GAL-4, -5, -6, and -10 were synergistically upregulated by VD3 in combination with LPS. These results suggest that VD3 enhances the immune immunity during LPS challenge by inducing the expression of chicken beta-defensin genes when birds are exposed to immune stressors.
- 相关文献
作者其他论文 更多>>
-
Myf5 and MyoG gene SNPs associated with Bian chicken growth trait
作者：Wei, Y.;Zhang, G. X.;Zhang, T.;Wang, J. Y.;Fan, Q. C.;Tang, Y.;Wei, Y.;Zhang, G. X.;Zhang, T.;Wang, J. Y.;Fan, Q. C.;Tang, Y.;Wei, Y.;Ding, F. X.;Zhang, L.
关键词：Bian chickens;Myf5;MyoG;Growth trait;SNP;Conjoint analysis
-
Molecular cloning and expression analysis of TRAF3 in chicken
作者：Yang, H. L.;Feng, Z. Q.;Zeng, S. Q.;Zhu, Q.;Liu, Y. P.;Li, S. M.
关键词：Chicken;Cloning;Gene expression;Newcastle disease virus;Tumor necrosis factor receptor-associated factor 3
-
Effects of vitamin D3 on expression of defensins, Toll-like receptors, and vitamin D receptor in liver, kidney, and spleen of Silky Fowl
作者：Li, S. M.;Ouyang, L. H.;Zhou, D. G.
关键词：vitamin D_3;induction;chicken;avian beta-defensins;real-time PCR
