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Effect of N-acetyl cysteine on enterocyte apoptosis and intracellular signalling pathways' response to oxidative stress in weaned piglets

文献类型： 外文期刊

作者： Zhu, Lihui 1 ; Cai, Xuan 1 ; Guo, Qi 1 ; Chen, Xiaolian 1 ; Zhu, Suwen 1 ; Xu, Jianxiong 1 ;

作者机构： 1.Shanghai Jiao Tong Univ, Sch Agr & Biol, Shanghai Key Lab Vet Biotechnol, Shanghai 200240, Peoples R China

2.Jiangxi Acad Agr Sci, Inst Anim Husb & Vet Sci, Nanchang 330200, Peoples R China

关键词： Piglets;Intestinal morphology;N-acetyl-L-cysteine;Apoptosis

期刊名称：BRITISH JOURNAL OF NUTRITION （ 影响因子：3.718； 五年影响因子：4.816 ）

ISSN： 0007-1145

年卷期： 2013 年 110 卷 11 期

页码：

收录情况： SCI

摘要： N-acetyl cysteine (NAC) has been widely used for preventing reactive oxygen species-induced damage. However, little is known as to whether dietary NAC supplementation would alleviate intestinal injury in weaned piglets. The present study evaluated the effect of NAC on enterocyte apoptosis and intracellular signalling pathways' response to weaning stress. The control piglets were normally suckling, and piglets in the weaning and NAC groups were fed the basal diet and basal + NAC diet from 14 to 25 d of age, respectively. Compared with the control piglets, weaning increased cortisol concentrations (P < 0.05), decreased superoxide dismutase and glutathione peroxidase activities (P < 0.05), increased malondialdehyde content (P < 0.05) in serum and enhanced enterocyte apoptosis index (AI) and concentrations of caspase-3, caspase-8 and caspase-9 (P < 0.05). Gene expression analyses indicated that weaning induced apoptosis via Fas signalling and mitochondrial pathways in weaned piglets. Dietary NAC supplementation decreased (P < 0.05) cortisol concentrations and the AI, increased (P < 0.05) antioxidant status in serum and alleviated histopathological changes in the intestine. It also inhibited Fas, caspase-3, caspase-8 and integrin alpha v beta 6 (alpha v beta 6) gene expressions in the NAC-treated piglets. However, no significant decrease (P < 0.10) in caspase-3, caspase-8 and caspase-9 concentrations was observed in the NAC group compared with the weaning group. In conclusion, weaning may induce enterocyte apoptosis via the activation of Fas-dependent and mitochondria-dependent apoptosis. Although NAC had no effect on caspase concentrations, it was clearly beneficial for preserving morphological integrity in weaned piglets via the regulation of cell apoptosis and the inhibition of Fas-dependent apoptosis and alpha v beta 6 expression.

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[1]Effects of dietary supplementation with N-acetyl cysteine on antioxidant capacities and the expression of inflammatory cytokines in weaned piglets. Guo, Qi,Cai, Xuan,Xu, Congcong,Luo, Zhen,Sheng, Yongshuai,Bao, Jian,Xu, Jianxiong,Guo, Qi,Cai, Xuan,Xu, Congcong,Luo, Zhen,Sheng, Yongshuai,Bao, Jian,Xu, Jianxiong,Chen, Xiaolian,Xu, Jianxiong. 2016

[2]X/XO or H2O2 induced IPEC-J2 cell as a new in vitro model for studying apoptosis in post-weaning piglets. Cai, Xuan,Zhu, Lihui,Sheng, Yongshuai,Guo, Qi,Bao, Jian,Xu, Jianxiong,Cai, Xuan,Zhu, Lihui,Sheng, Yongshuai,Guo, Qi,Bao, Jian,Xu, Jianxiong,Chen, Xiaolian.

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