文献类型： 外文期刊

作者： Huang, Huaxing ¹ ; Ji, Hongli ³ ; Ju, Song ¹ ; Lin, Wei ⁴ ; Li, Jing ¹ ; Lv, Xuanrui ¹ ; Lin, Lixian ¹ ; Guo, Lijin ⁷ ; Qiu, Dongliang ⁵ ; Yan, Jianyong ² ; Ma, Xiaokai ¹ ;

作者机构： 1.Fujian Agr & Forestry Univ, Haixia Inst Sci & Technol, Ctr Genom & Biotechnol, Sch Future Technol, Fuzhou, Peoples R China

2.Yuanshan Inst Bougainvillea Longhai, Zhangzhou, Peoples R China

3.Jiangxi Acad Agr Sci, Vegetable & Flower Inst, Nanchang, Peoples R China

4.Fujian Agr & Forestry Univ, Coll Forestry, Fuzhou, Peoples R China

5.Fujian Agr & Forestry Univ, Coll Hort, Fuzhou, Peoples R China

6.Fujian Agr & Forestry Univ, Coll Life Sci, Fuzhou, Peoples R China

7.Fujian Agr & Forestry Univ, Int Magnesium Inst, Haixia Inst Sci & Technol, Sch Future Technol, Fuzhou, Peoples R China

8.Fujian Agr & Forestry Univ, Coll Landscape Architecture, Key Lab Orchid Conservat & Utilizat, Natl Forestry & Grassland Adm, Fuzhou, Peoples R China

关键词： pan-transcriptome; Bougainvillea bracts; color variation; co-expression network; gene regulation

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：5.6； 五年影响因子：6.8 ）

ISSN： 1664-462X

年卷期： 2022 年 13 卷

页码：

收录情况： SCI

摘要： Bracts are the metamorphic non-flower organ in angiosperm plants. The variation of the color and shape of bracts was found to be neo-functionalized (i.e., similar to petals), garnering research interest as a pollinator attractor. Bougainvillea is known for its specialized, large, and colorful bracts, which contrast with its tiny colorless flowers. As a plant whose bracts vary greatly in terms of coloration, the molecular mechanisms for Bougainvillea bract coloration and polychroism are largely unknown. The lack of genomic information for Bougainvillea largely hinders studies into the evolution and genetic basis of bract color variation. In this study, a pan-transcriptome of bracts obtained from 18 Bougainvillea glabra accessions was employed to investigate the global population-level germplasm kinship and the gene regulation network for bract color variation. Our results showed that the bracts of B. glabra accessions have largely differentiated International Commission on Illumination (CIE) L-a-b values. Moreover, germplasm kinship detected using principal component analysis, phylogeny, and admixture analysis showed three optimal subgroups, two of them distinctly clustered, which were not directly correlated with bract color variation at the population level. Differentially expressed genes (DEGs) between accessions of high vs. low L-a-b values revealed several considerable upregulated genes related to bract color L-a-b variation. A weighted gene co-expression network was constructed, and eight co-expressed regulation modules were identified that were highly correlated with variation in bract CIE L-a-b color values. Several candidate DEGs and co-expressed hub genes (e.g., GERD, SGR, ABCA3, GST, CYP76AD1, CYP76C, and JAZ) that were tightly associated with bract color variation were eventually determined responsible for L-a-b colorations, which might be the core regulation factors contributing to the B. glabra bract color variation. This study provides valuable insights into the research on germplasm kinship, population-level pan-transcriptome expression profiles, and the molecular basis of color variation of key innovative bracts in horticultural Bougainvillea.