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Functional characterization of murB-porABCD operon for polyamine uptake and peptidoglycan synthesis in Streptococcus suis

文献类型： 外文期刊

作者： Liu, Wanquan 1 ; Tan, Meifang 2 ; Zhang, Chunyan 1 ; Xu, Zhuofei 1 ; Li, Lu 1 ; Zhou, Rui 1 ;

作者机构： 1.Huazhong Agr Univ, Coll Vet Med, State Key Lab Agr Microbiol, Wuhan 430070, Peoples R China

2.Jiangxi Acad Agr Sci, Inst Anim Husb & Vet Sci, Nanchang, Jiangxi, Peoples R China

3.Cooperat Innovat Ctr Sustainable Pig Prod, Wuhan 430070, Peoples R China

4.Minist Sci & Technol China, Int Res Ctr Anim Dis, Wuhan 430070, Peoples R China

关键词： Streptococcus suis; Spermidine; Spermine; Putrescine; Polyamine uptake; Peptidoglycan (PG)

期刊名称：MICROBIOLOGICAL RESEARCH （ 影响因子：5.415； 五年影响因子：6.038 ）

ISSN： 0944-5013

年卷期： 2018 年 207 卷

页码：

收录情况： SCI

摘要： Spermidine (Spd), spermine (Spm), and putrescine (Put), which are the most widely distributed cellular poly amines, are essential for normal growth and multiplication of both eukaryotic and prokaryotic cells. In this study, we identified the only putative polyamine transport system PotABCD in Streptococcus suis, a worldwide zoonotic Gram-positive pathogen causing lethal infections in humans and pigs. It was discovered that S. suis could uptake polyamines preferably Spd and Spm. By constructing a potA deleted mutant, we confirmed that PotABCD was responsible for polyamine uptake, and PotD bound to the protein of polyamines. The four PotABCD genes were co-transcribed with murB, a gene involved in peptidoglycan (PG) synthesis. Furthermore the roles of polyamine transport system in maintaining the PG structure were detected to understand the biological significance of this co-transcription. In contrast to the wild type, the mutant ApotA exhibited elongated chain length and abnormal cell division morphology. Phenotypic changes were attributed to be the up-regulation of genes involved in PG synthesis and hydrolysis in ApotA. Additionally, polyamines functioned not only as feedback regulators of PotA by inhibiting PotA activity but also as regulators on potABCD and genes involved in PG synthesis. This study reveals the functions of PotABCD in polyamine transport and the regulatory roles of polyamines in PG synthesis. Results provide new insights into the machineries contributing to normal growth and cell division of S. suis.

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