您好，欢迎访问江西省农业科学院 机构知识库！

DNA template-mediated click chemistry-based portable signal-on sensor for ochratoxin A detection

文献类型： 外文期刊

作者： Qiu, Suyan 1 ; Yuan, Lijuan 1 ; Wei, Yihua 1 ; Zhang, Dawen 1 ; Chen, Qinglong 1 ; Lin, Zhenyu 2 ; Luo, Linguang 1 ;

作者机构： 1.Jiangxi Acad Agr Sci, Inst Qual & Safety & Stand Agr Prod Res, Nanchang 330200, Jiangxi, Peoples R China

2.Fuzhou Univ, Dept Chem, MOE Key Lab Anal & Detect Food Safety, Fujian Prov Key Lab Anal & Detect Technol Food Sa, Fuzhou 350002, Fujian, Peoples R China

关键词： DNA template-mediated click chemistry; Personal glucose meter; Ochratoxin A; Copper(I) catalyzed azide-alkyne cycloaddition; Magnetic beads

期刊名称：FOOD CHEMISTRY （ 影响因子：7.514； 五年影响因子：7.516 ）

ISSN： 0308-8146

年卷期： 2019 年 297 卷

页码：

收录情况： SCI

摘要： A novel signal-on portable sensing system has been developed for OTA detection using personal glucose meter (PGM) as signal transducer. In the study, we explore the potential of using a short dsDNA as template to trigger the "click" ligation of two DNA strands, further improve the stability of DNA strand on the magnetic beads (MBs) surface, and thereby reduce the background signal. Compared with no "click" ligation, the background signal decreases 7.5 times. Both the sensitivity and selectivity are greatly promoted. A high sensitivity with OTA detection down to 72 pg/mL is achieved, which is comparable with several existing detectors, such as fluorescence-based detectors and electrochemical detectors. The feasibility of the strategy in real samples is well verified and evaluated by detecting OTA in feed samples, indicating the potential application in the food safety field.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：江西省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台