DNA template-mediated click chemistry-based portable signal-on sensor for ochratoxin A detection
文献类型： 外文期刊
作者： Qiu, Suyan 1 ; Yuan, Lijuan 1 ; Wei, Yihua 1 ; Zhang, Dawen 1 ; Chen, Qinglong 1 ; Lin, Zhenyu 2 ; Luo, Linguang 1 ;
作者机构： 1.Jiangxi Acad Agr Sci, Inst Qual & Safety & Stand Agr Prod Res, Nanchang 330200, Jiangxi, Peoples R China
2.Fuzhou Univ, Dept Chem, MOE Key Lab Anal & Detect Food Safety, Fujian Prov Key Lab Anal & Detect Technol Food Sa, Fuzhou 350002, Fujian, Peoples R China
关键词： DNA template-mediated click chemistry; Personal glucose meter; Ochratoxin A; Copper(I) catalyzed azide-alkyne cycloaddition; Magnetic beads
期刊名称：FOOD CHEMISTRY （ 影响因子：7.514； 五年影响因子：7.516 ）
ISSN： 0308-8146
年卷期： 2019 年 297 卷
页码：
收录情况： SCI
摘要： A novel signal-on portable sensing system has been developed for OTA detection using personal glucose meter (PGM) as signal transducer. In the study, we explore the potential of using a short dsDNA as template to trigger the "click" ligation of two DNA strands, further improve the stability of DNA strand on the magnetic beads (MBs) surface, and thereby reduce the background signal. Compared with no "click" ligation, the background signal decreases 7.5 times. Both the sensitivity and selectivity are greatly promoted. A high sensitivity with OTA detection down to 72 pg/mL is achieved, which is comparable with several existing detectors, such as fluorescence-based detectors and electrochemical detectors. The feasibility of the strategy in real samples is well verified and evaluated by detecting OTA in feed samples, indicating the potential application in the food safety field.
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