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Microarray detection and qPCR screening of potential biomarkers of Folsomia Candida (Collembola: Isotomidae) exposed to Bt proteins (Cry1Ab and Cry1Ac)

文献类型： 外文期刊

作者： Yuan, Yiyang 1 ; Krogh, Paul Henning 2 ; Bai, Xue 3 ; Roelofs, Dick 4 ; Chen, Fajun 5 ; Zhu-Salzman, Keyan 6 ; Liang, 1 ;

作者机构： 1.Chinese Acad Sci, Inst Zool, State Key Lab Integrated Management Pest & Rodent, Beijing 100101, Peoples R China

2.Aarhus Univ, Dept Biosci, DK-8600 Silkeborg, Denmark

3.Southwest Univ Nationalities, Coll Life Sci & Technol, Chengdu 610041, Peoples R China

4.Vrije Univ Amsterdam, Inst Ecol Sci, NL-1081 HV Amsterdam, Netherlands

5.Nanjing Agr Univ, Dept Entomol, Coll Plant Protect, Nanjing 210095, Peoples R China

6.Texas A&M Univ, Dept Entomol, College Stn, TX 77843 USA

7.Jiangxi Acad Agr Sci, Inst Plant Protect, Nanchang 330200, Peoples R China

关键词： Non-target organism;Folsomia Candida;Bt proteins;Microarray

期刊名称：ENVIRONMENTAL POLLUTION （ 影响因子：8.071； 五年影响因子：8.35 ）

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年卷期：

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收录情况： SCI

摘要： The impact of fir proteins on non-target arthropods is less understood than their effects on target organisms where the mechanism of toxic action is known. Here, we report the effects of two Bt proteins, CrylAb and CrylAc, on gene expression in the non-target collembolan, Folsomia Candida. A customized microarray was used to study gene expression in F. Candida specimens that were exposed to CrylAb and CrylAc. All selected transcripts were subsequently confirmed by qPCR. Eleven transcripts were finally verified, and three of them were annotated. The responses of all eleven transcripts were tested in specimens for both CrylAb and CrylAc at a series of concentrations. These transcripts were separated into two and three groups for CrylAb and CrylAc, respectively, depend on their expression levels. However, those eleven transcripts did not respond to the Bt proteins in fit-rice residues.

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